Anti-CCDC125 antibody (ab122750)
Key features and details
- Rabbit polyclonal to CCDC125
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CCDC125 antibody -
Description
Rabbit polyclonal to CCDC125 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: VLNQRYLEAL AMLDIKQQKM AQENMCCDKS GFAEASGLEL AVLGACLCHG PGGNPCSCAR MAASTRKLLL QLKQELEILQ KSKEEAYVMA DAFRIAFE, corresponding to amino acids 234-331 of Human CCDC125.
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Positive control
- Human colon tissue; U 2 OS cells; RT 4, U 251 MG, Human liver and tonsil lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
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Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization. |
Target
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Function
May be involved in the regulation of cell migration. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 202243 Human
- Omim: 613781 Human
- SwissProt: Q86Z20 Human
- Unigene: 654850 Human
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Alternative names
- CC125_HUMAN antibody
- CCDC125 antibody
- Coiled-coil domain-containing protein 125 antibody
see all
Images
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ab122750 at 1/20 dilution, staining CCDC125 in Paraffin-embedded Human colon tissue by Immunohistochemistry.
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All lanes : Anti-CCDC125 antibody (ab122750) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human Plasma
lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lyaste
Developed using the ECL technique. -
ab122750 at 4 µg/ml staining CCDC125 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122750 has not yet been referenced specifically in any publications.