Overview

  • Product name
    Anti-CCDC125 antibody
  • Description
    Rabbit polyclonal to CCDC125
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: VLNQRYLEAL AMLDIKQQKM AQENMCCDKS GFAEASGLEL AVLGACLCHG PGGNPCSCAR MAASTRKLLL QLKQELEILQ KSKEEAYVMA DAFRIAFE, corresponding to amino acids 234-331 of Human CCDC125.

  • Positive control
    • Human colon tissue; U 2 OS cells; RT 4, U 251 MG, Human liver and tonsil lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

Target

Images

  • ab122750 at 1/20 dilution, staining CCDC125 in Paraffin-embedded Human colon tissue by Immunohistochemistry.
  • All lanes : Anti-CCDC125 antibody (ab122750) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lyaste

    Developed using the ECL technique
  • ab122750 at 4 µg/ml staining CCDC125 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).

References

ab122750 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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