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Rabbit polyclonal to CCDC21
Predicted to work with:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 675-725 of Human CCDC21 (NP_073615.2).
Jurkat, HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7-8
Concentration information loading...
Immunogen affinity purified
ab117782 was affinity purified using an epitope specific to CCDC21 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
Use at 2-10 µg/mg of lysate.
Belongs to the CEP85 family.
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Nucleus > nucleolus. Localizes to centrosomes and nucleolus in interphase. Upon entry into mitosis, relocates from nucleolus and accumulates at spindle poles.
Information by UniProt
Centrosomal protein of 85 kDa antibody
Western blot - Anti-CCDC21 antibody (ab117782)
All lanes : Anti-CCDC21 antibody (ab117782) at 0.1 µg/ml Lane 1 : 293T whole cell lysates at 50 µg Lane 2 : 293T whole cell lysates at 15 µg Lane 3 : Jurkat whole cell lysates at 50 µg Lane 4 : HeLa whole cell lysates at 50 µg Developed using the ECL technique. Predicted band size: 86 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-CCDC21 antibody (ab117782)
Detection of CCDC21 by Western Blot of Immunprecipitate.
ab117782 at 1µg/ml staining CCDC21 in 293T whole cell lysate immunoprecipitated using ab117782 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"