Overview

  • Product name
  • Description
    Rabbit polyclonal to CCDC21
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 675-725 of Human CCDC21 (NP_073615.2).

  • Positive control
    • Jurkat, HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab117782 was affinity purified using an epitope specific to CCDC21 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Sequence similarities
    Belongs to the CEP85 family.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Nucleus > nucleolus. Localizes to centrosomes and nucleolus in interphase. Upon entry into mitosis, relocates from nucleolus and accumulates at spindle poles.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCDC21 antibody
    • Centrosomal protein of 85 kDa antibody
    • CEP85 antibody
    • CEP85_HUMAN antibody
    • Coiled-coil domain containing 21 antibody
    • Coiled-coil domain-containing protein 21 antibody
    • DKFZp434L0117 antibody
    • DKFZp434P232 antibody
    • FLJ13976 antibody
    • FLJ22000 antibody
    • OTTHUMP00000003454 antibody
    • OTTHUMP00000003455 antibody
    • OTTHUMP00000003456 antibody
    • OTTHUMP00000003457 antibody
    see all

Images

  • All lanes : Anti-CCDC21 antibody (ab117782) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysates at 50 µg
    Lane 2 : 293T whole cell lysates at 15 µg
    Lane 3 : Jurkat whole cell lysates at 50 µg
    Lane 4 : HeLa whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 86 kDa


    Exposure time : 3 minutes
  • Detection of CCDC21 by Western Blot of Immunprecipitate.
    ab117782 at 1µg/ml staining CCDC21 in 293T whole cell lysate immunoprecipitated using ab117782 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab117782 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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