The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
Belongs to the CEP85 family.
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Nucleus > nucleolus. Localizes to centrosomes and nucleolus in interphase. Upon entry into mitosis, relocates from nucleolus and accumulates at spindle poles.
Lane 1: Staining of CCDC21 in 293T whole cell lysate immunoprecipitated using ab117802 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Predicted band size : 86 kDa.
Exposure time: 30 seconds.