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Rabbit polyclonal to CCDC23
antigen: DPPARKEKTK VKESVSRVEK AKQKSAQQEL KQRQRAEIYA LNRVMTELEQ QQFDEFCKQM QPPGE, corresponding to amino acids 2-66 of Human CCDC23 (Q8N300).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Belongs to the CCDC23 family.
Information by UniProt
- CCD23_HUMAN antibody
- ccdc23 antibody
- Coiled-coil domain-containing protein 23 antibody
Immunocytochemistry/ Immunofluorescence - Anti-CCDC23 antibody (ab121282)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121282 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC23 antibody (ab121282)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human esophagus tissue labelled with ab121282 at 1/40 dilution.
Western blot - Anti-CCDC23 antibody (ab121282)
All lanes : Anti-CCDC23 antibody (ab121282) at 1/250 dilution
Lane 1 : Marker
Lane 2 : Vector transfected HEK293T cell lysate
Lane 3 : HEK293T cell lysate transfected with CCDC23 and C-terminal myc-DDK tag
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"