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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/250 - 1/500.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CCDC34 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-CCDC34 antibody (ab122396)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus, nucleoli and nuclear membrane. Recommended concentration of ab122396 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-CCDC34 antibody (ab122396)
All lanes : Anti-CCDC34 antibody (ab122396) at 1/250 dilution Lane 1 : RT4 cell lysate Lane 2 : U251 MG cell lysate Lane 3 : Human Plasma cell lysate Lane 4 : Liver cell lysate Lane 5 : Tonsil cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC34 antibody (ab122396)
, at 1/500, staining CCDC34 in paraffin embedded Human cerebral cortex tissue by immunohistochemistry.
References for Anti-CCDC34 antibody (ab122396)
This product has been referenced in:
et al. Over-expression of ASIC1a promotes proliferation via activation of the ß-catenin/LEF-TCF axis and is associated with disease outcome in liver cancer. Oncotarget 8:25977-25988 (2017).
Read more (PubMed: 27462920) »
et al. CCDC34 is up-regulated in bladder cancer and regulates bladder cancer cell proliferation, apoptosis and migration. Oncotarget 6:25856-67 (2015).
Read more (PubMed: 26312564) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"