Overview

  • Product name
  • Description
    Rabbit polyclonal to CCDC34
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    internal sequence amino acids 92-191 (DEEDVDEDAH DSEAKVASLR GMELQGCAST QVESENNQEE QKQVRLPESR LTPWEVWFIG KEKEERDRLQ LKALEELNQQ LEKRKEMEER EKRKIIAEEK) of the Human CCDC34 protein (Q96HJ3).

  • Positive control
    • IHC-P: Human cerebral cortex tissue WB: RT4 and U251 MG cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Involvement in disease
    Note=A chromosomal aberration involving CCDC34 is found in a patient with hamartoma of the retinal pigment epithelium and retina. Translocation t(11;18) (p13;p11.2).
  • Information by UniProt
  • Database links
  • Alternative names
    • CCD34_HUMAN antibody
    • Ccdc34 antibody
    • Coiled-coil domain-containing protein 34 antibody
    • L15 antibody
    • NY-REN-41 antibody
    • RAMA3 antibody
    • Renal carcinoma antigen NY-REN-41 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus, nucleoli and nuclear membrane. Recommended concentration of ab122396 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-CCDC34 antibody (ab122396) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human Plasma cell lysate
    Lane 4 : Liver cell lysate
    Lane 5 : Tonsil cell lysate
  • ab122430, at 1/500, staining CCDC34 in paraffin embedded Human cerebral cortex tissue by immunohistochemistry.

References

This product has been referenced in:
  • Jin C  et al. Over-expression of ASIC1a promotes proliferation via activation of the ß-catenin/LEF-TCF axis and is associated with disease outcome in liver cancer. Oncotarget 8:25977-25988 (2017). Read more (PubMed: 27462920) »
  • Gong Y  et al. CCDC34 is up-regulated in bladder cancer and regulates bladder cancer cell proliferation, apoptosis and migration. Oncotarget 6:25856-67 (2015). WB, IHC . Read more (PubMed: 26312564) »

See all 2 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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