The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Required for assembly of dynein regulatory complex (DRC) and inner dynein arm complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Not required for outer dynein arm complexes assembly.
Mainly expressed in nasal brushings and, to a lesser extent, in lungs and testis.
Involvement in disease
Defects in CCDC39 are the cause of primary ciliary dyskinesia type 14 (CILD14) [MIM:613807]. A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
Belongs to the CCDC39 family.
Cytoplasm > cytoskeleton > cilium axoneme. CCDC40 is required for localization to axonemes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchus tissue labelling CCDC39 with ab122269. Staining shows strong positivity in cilia of respiratory epithelial cells.
Western blot - Anti-CCDC39 antibody (ab122269)
All lanes : Anti-CCDC39 antibody (ab122269) at 1/250 dilution
Lane 1 : RT-4 lysate Lane 2 : U251-MG lysate Lane 3 : Human plasma lysate Lane 4 : Human liver lysate Lane 5 : Human tonsil lysate