- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-CCDC40 antibody
-
DescriptionRabbit polyclonal to CCDC40
-
Host speciesRabbit
-
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
-
Species reactivityReacts with: Human
-
Immunogen
antigen sequence, corresponding to amino acids 19-104 (SEGEKEGNNE SHMVSPPEKD DGQKGEEAVG STEHPEEVTT QAEAAIEEGE VETEGEAAVE GEEEAVSYGD AESEEEYYYT ETSSPE) of Human CCDC40.
-
Positive control
- Human urinary bladder tissue; RT4, U251 MG, Human liver and tonsil lysates.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab121727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
|
| WB | 1/250 - 1/500. | |
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
FunctionRequired for assembly of dynein regulatory complex (DRC) and inner dynein arm complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Not required for outer dynein arm complexes assembly. Required for axonemal recruitment of CCDC39.
-
Involvement in diseaseDefects in CCDC40 are the cause of primary ciliary dyskinesia type 15 (CILD15) [MIM:613808]. A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
-
Sequence similaritiesBelongs to the CCDC40 family.
-
Cellular localizationCytoplasm. Cell projection > cilium. Localizes to cytoplasm and motile cilium.
- Information by UniProt
-
Database links
- Entrez Gene: 55036 Human
- Omim: 613799 Human
- SwissProt: Q4G0X9 Human
- Unigene: 202542 Human
-
Alternative names
- CCD40_HUMAN antibody
- ccdc 40 antibody
- CCDC40 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-CCDC40 antibody (ab121727)Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoskeleton (microtubules). Recommended concentration of ab121727 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC40 antibody (ab121727)ab121727 at 1/500 dilution staining CCDC40 in Paraffin-embedded Human urinary bladder tissue by Immunohistochemistry. -
Western blot - Anti-CCDC40 antibody (ab121727)All lanes : Anti-CCDC40 antibody (ab121727) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver tissue lysate
Lane 5 : Human Tonsil tissue lysate
Developed using the ECL technique.
Protocols
References
ab121727 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"