Overview

  • Product name
    Anti-CCDC40 antibody
  • Description
    Rabbit polyclonal to CCDC40
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 19-104 (SEGEKEGNNE SHMVSPPEKD DGQKGEEAVG STEHPEEVTT QAEAAIEEGE VETEGEAAVE GEEEAVSYGD AESEEEYYYT ETSSPE) of Human CCDC40.

  • Positive control
    • Human urinary bladder tissue; RT4, U251 MG, Human liver and tonsil lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Required for assembly of dynein regulatory complex (DRC) and inner dynein arm complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Not required for outer dynein arm complexes assembly. Required for axonemal recruitment of CCDC39.
  • Involvement in disease
    Defects in CCDC40 are the cause of primary ciliary dyskinesia type 15 (CILD15) [MIM:613808]. A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
  • Sequence similarities
    Belongs to the CCDC40 family.
  • Cellular localization
    Cytoplasm. Cell projection > cilium. Localizes to cytoplasm and motile cilium.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCD40_HUMAN antibody
    • ccdc 40 antibody
    • CCDC40 antibody
    • Coiled coil domain containing 40 antibody
    • Coiled-coil domain-containing protein 40 antibody
    • FLJ20753 antibody
    • FLJ32021 antibody
    • KIAA1640 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoskeleton (microtubules). Recommended concentration of ab121727 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121727 at 1/500 dilution staining CCDC40 in Paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
  • All lanes : Anti-CCDC40 antibody (ab121727) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver tissue lysate
    Lane 5 : Human Tonsil tissue lysate

    Developed using the ECL technique.

References

ab121727 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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