Overview

  • Product name
  • Description
    Rabbit polyclonal to CCDC52
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 475-525 of Human CCDC52 according to NP_653319.1.

  • Positive control
    • Whole cell lysate from 293T and HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 96 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-CCDC52 antibody (ab117820) at 0.04 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 96 kDa


    Exposure time : 3 minutes
  • Detection of Human CCDC52 by Immunoprecipitation in whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded) using ab117820 at 6 µg/mg. Subsequent WB detection was done using ab117820 at 1 µg/ml. Detection: chemiluminescence with exposure time of 10 second.

References

ab117820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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