Overview

  • Product name
    Anti-CCDC65 antibody
  • Description
    Rabbit polyclonal to CCDC65
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SSVLTPKEQE GIQKNNLEEL TEELTKVMVD YIGMENFWKR YNKVKLEQLS LQHRRAQLLD INGKLREMLK QYLDGISVSD EVLSQ, corresponding to amino acids 368-452 of Human CCDC65 (Q8IXS2).

  • Positive control
    • Human prostate tissue. Human Plasma lysate. Human tonsil tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Tissue specificity
    Highly expressed in adult testis, in spermatocytes and spermatids. Also observed in spermatogonia. Not detected in Leydig cells, nor in fetal testis (at protein level).
  • Information by UniProt
  • Database links
  • Alternative names
    • CCD65_HUMAN antibody
    • CCDC65 antibody
    • Coiled coil domain containing 65 antibody
    • Coiled-coil domain-containing protein 65 antibody
    • NYD-SP28 antibody
    • Testis development protein NYD-SP28 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab122482 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122482 at 1/200 dilution staining CCDC65 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • All lanes : Anti-CCDC65 antibody (ab122482) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique

References

ab122482 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab122482.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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