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Rabbit polyclonal to CCDC75
Mouse, Rat, Human
antigen sequence: KSGIGHEASL KRKAEEKLES YRKKIHMKNQ AEEKAAEQFR MRLKNKQDEM KLEGDLRRSQ RACQQLDVQK NIQVPREAWY WL, corresponding to amino acids 107-188 of Human CCDC75
- Human testis tissue; RT4, U251 MG, Human Liver and Tonsil lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/250 - 1/500.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Contains 1 G-patch domain.
Information by UniProt
- CCD75_HUMAN antibody
- CCDC75 antibody
- CENP Y antibody
Immunocytochemistry/ Immunofluorescence - Anti-CCDC75 antibody (ab122451)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus. Recommended concentration of ab122451 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC75 antibody (ab122451)
ab122451, at 1/200, staining CCDC75 in paraffin embedded Human testis tissue by immunohistochemistry.
Western blot - Anti-CCDC75 antibody (ab122451)
All lanes : Anti-CCDC75 antibody (ab122451) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Liver lysate
Lane 5 : Tonsil lysate
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"