All lanes : Anti-CCDC82 antibody (ab86779) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique
Predicted band size : 64 kDa
Exposure time : 3 minutes
Immunoprecipitation - CCDC82 antibody (ab86779)
HeLa whole cell lysate (1 mg) was immunoprecipitated with 10 µg ab86779 (lane 1) or IgG control (lane 2). 20% of the immunoprecipitate was subjected to Western blotting and labelled with ab86779 at 0.1 µg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds.