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Rabbit polyclonal to CCDC85A
antigen sequence: RHPHPGSSPE TLPKHVLSGS PEHFQKHRSG SSPEHARHSG GSPEHLQKHA LGGSLEHLPR ARGTSPEHLK QHYGGSPDHK HGGGSGGS, corresponding to internal sequence amino acids 294-381 of Human CCDC85A.
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the CCDC85 family.
Information by UniProt
- CC85A_HUMAN antibody
- Ccdc85a antibody
- Coiled-coil domain containing 85A antibody
Immunocytochemistry/ Immunofluorescence - Anti-CCDC85A antibody (ab122831)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122831 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC85A antibody (ab122831)
ab122831 at 1/200 dilution staining CCDC85A in Paraffin-embedded Human rectum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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