Overview

  • Product name
    Anti-CCDC89 antibody
  • Description
    Rabbit polyclonal to CCDC89
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    amino acids 249-347 (KAEETHSSLS QELQARLQTV TREKEELLQL SIERGKVLQN KQAEICQLEE KLEIANEDRK HALERFEQEA VAVDSNLRVR ELQRKVDGIQ KAYDELRLQ) of the Human CCDC89 protein (Q8N998).

  • Positive control
    • Human pancreas tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities
    Belongs to the CCDC89 family.
  • Cellular localization
    Cytoplasm. Nucleus. Uniformly distributed within the cell, but becomes recruited to the nucleus upon binding to HEY1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bc8 orange-interacting protein antibody
    • BOIP antibody
    • CCD89_HUMAN antibody
    • Ccdc89 antibody
    • Coiled coil domain containing 89 antibody
    • Coiled-coil domain-containing protein 89 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows no positivity. Recommended concentration of ab122550 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122550, at 1:200, staining CCDC89 in Human pancreas tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
  • All lanes : Anti-CCDC89 antibody (ab122550) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal proprietary tag (~3.1 kDa) in mammalian HEK293T cells

References

ab122550 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab122550.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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