Anti-CCDC90A antibody (ab86335)

Overview

  • Product nameAnti-CCDC90A antibody
    See all CCDC90A primary antibodies
  • Description
    Rabbit polyclonal to CCDC90A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cat, Dog
  • Immunogen

    Synthetic peptide from within residues ELHQLKQQVM DEVIKVRTDT KLDFNLEKSR VKELYSLNEK KLLELRTEIV, corresponding to internal sequence amino acids 252-301 of Human CCDC90A (NP_001026883)

  • Positive control
    • Human fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-CCDC90A antibody images

  • Anti-CCDC90A antibody (ab86335) at 1 µg/ml + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 40 kDa

References for Anti-CCDC90A antibody (ab86335)

ab86335 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Brain tissue homogenate)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 50 µg
Specification Brain tissue homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Aug 19 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (4-12& BIS-TRIS)
Sample Human Cell lysate - whole cell (fibroblasts)
Specification fibroblasts
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Feb 20 2014

The immunogen identity with the mouse protein is 92%.

The immunogen has 92% identity with the rat protein, so although cross-reactivity with rat CCDC90A is likely, there is a chance that most of the reactivity with the human protein is due to recognition of an epitope that is not present on the rat protei...

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Gracias por contactarnos.

La homología de secuencias entre el inmunógeno del anticuerpo y la proteína en rata es del 92%. Por tanto, es muy probable que el anticuerpo de buenos resultados en esta especie, en las aplicacio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"