Overview

  • Product nameAnti-CCK4 antibody
    See all CCK4 primary antibodies
  • Description
    Rabbit polyclonal to CCK4
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the amino terminus of human CCK4.

  • Positive control
    • Human, mouse or rat colon membrane lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab62074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 118 kDa.
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr 1/500.

Target

  • FunctionInactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
  • Tissue specificityHighly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • DomainThe protein kinase domain is predicted to be catalytically inactive.
  • Post-translational
    modifications
    MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway.
  • Cellular localizationMembrane. Cell junction. Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 8430404F20Rik antibody
    • CCK 4 antibody
    • CCK-4 antibody
    • Colon carcinoma kinase 4 antibody
    • Human colon carcinoma kinase 4 (CCK4) mRNA complete cds antibody
    • Inactive tyrosine-protein kinase 7 antibody
    • mPTK7/CCK4 antibody
    • OTTHUMP00000217016 antibody
    • Protein tyrosine kinase PTK 7 antibody
    • Protein-tyrosine kinase 7 antibody
    • Pseudo tyrosine kinase receptor 7 antibody
    • PTK 7 antibody
    • PTK7 antibody
    • PTK7 protein tyrosine kinase 7 antibody
    • PTK7 protein tyrosine kinase antibody
    • PTK7_HUMAN antibody
    • Serum response factor antibody
    • Srf antibody
    • Tyrosine-protein kinase-like 7 antibody
    see all

Anti-CCK4 antibody images

  • ab62074 staining CCK4 in E9.5 mouse embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 5% donkey serum) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    Image shows sagittal section of E9.5 mouse embryo. Neuroectoderm and underlying mesoderm labelled with ab62074 in green. DAPI stained nuclei in blue.

    See Abreview

  • All lanes : Anti-CCK4 antibody (ab62074) at 1 µg/ml

    Lane 1 : Human colon tissue lysate
    Lane 2 : Mouse colon tissue lysate
    Lane 3 : Rat colon tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    anti-rabbit IgG

    Predicted band size : 118 kDa
  • ab62074 at 5 µg/ml staining CCK4 in human colon tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

References for Anti-CCK4 antibody (ab62074)

ab62074 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (E9.5 mouse embryo section)
Specification E9.5 mouse embryo section
Permeabilization Yes - PBST (PBS/0.1% v/v TritonX100 (sigma))
Fixative Paraformaldehyde
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Submitted Aug 08 2014

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