For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
A 15 amino acid peptide from near the amino terminus of human CCK4.
Our Abpromise guarantee covers the use of ab62074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 118 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
ab62074 staining CCK4 in E9.5 mouse embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 5% donkey serum) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Image shows sagittal section of E9.5 mouse embryo. Neuroectoderm and underlying mesoderm labelled with ab62074 in green. DAPI stained nuclei in blue.
ab62074 at 5 µg/ml staining CCK4 in human colon tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
ab62074 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"