Overview

  • Product nameAnti-CCK4 antibody
    See all CCK4 primary antibodies
  • Description
    Mouse monoclonal to CCK4
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 36-146 of Human CCK4, with a 26kDa tag. NP_002812.

  • Positive control
    • Recombinant protein, A431 cell lysate and HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 118 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionInactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
  • Tissue specificityHighly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • DomainThe protein kinase domain is predicted to be catalytically inactive.
  • Post-translational
    modifications
    MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway.
  • Cellular localizationMembrane. Cell junction. Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 8430404F20Rik antibody
    • CCK 4 antibody
    • CCK-4 antibody
    • Colon carcinoma kinase 4 antibody
    • Human colon carcinoma kinase 4 (CCK4) mRNA complete cds antibody
    • Inactive tyrosine-protein kinase 7 antibody
    • mPTK7/CCK4 antibody
    • OTTHUMP00000217016 antibody
    • Protein tyrosine kinase PTK 7 antibody
    • Protein-tyrosine kinase 7 antibody
    • Pseudo tyrosine kinase receptor 7 antibody
    • PTK 7 antibody
    • PTK7 antibody
    • PTK7 protein tyrosine kinase 7 antibody
    • PTK7 protein tyrosine kinase antibody
    • PTK7_HUMAN antibody
    • Serum response factor antibody
    • Srf antibody
    • Tyrosine-protein kinase-like 7 antibody
    see all

Anti-CCK4 antibody images

  • Anti-CCK4 antibody (ab88077) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 118 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-CCK4 antibody (ab88077) at 1/500 dilution + A431 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 118 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • ab88077 at 10µg/ml staining CCK4 in Hela cells.
  • Detection limit for ab88077 is approximately 1ng/ml as a capture antibody.

References for Anti-CCK4 antibody (ab88077)

ab88077 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"