Overview

  • Product name
  • Description
    Goat polyclonal to CCKAR
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide:

    C-FEASQKKSAKERKPST

    , corresponding to internal sequence amino acids 242-257 of Human CCKAR (NP_000721.1).

  • Positive control
    • Human Pancreas tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCo2

Properties

Applications

Our Abpromise guarantee covers the use of ab77269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Preliminary experiments in Human Pancreas lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration. Antibody detection limit for peptide ELISA 1:4000
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Relevance
    The cholecystokinin (CCK) family of peptide hormones have been implicated in numerous important physiologic events. These appear to be mediated through 2 general classes of receptors, A (CCKAR)and B (CCKBR), based on their binding affinities for CCK/gastrin family peptides. Through binding to class A receptors, CCK is a major physiologic mediator of gallbladder contraction and pancreatic enzyme secretion. It appears to play a role in slowing gastric emptying, relaxation of the sphincter of Oddi, and potentiation of insulin secretion. Further, it has been implicated as a mediator of pancreatic growth and tumorigenesis. Class A receptors have also been described in the anterior pituitary, myenteric plexus, and regions of the central nervous system, where they have been implicated in the pathogenesis of feeding disorders, Parkinson disease, schizophrenia, and drug addiction.
  • Cellular localization
    Integral membrane protein
  • Database links
  • Alternative names
    • CCK A antibody
    • CCK A receptor antibody
    • CCK AR antibody
    • CCK-A receptor antibody
    • CCK1 R antibody
    • CCK1-R antibody
    • CCK1R antibody
    • CCKA antibody
    • CCKA receptor antibody
    • CCKR Type A antibody
    • CCKRA antibody
    • Cholecystokinin 1 receptor antibody
    • Cholecystokinin A receptor antibody
    • Cholecystokinin receptor type A antibody
    • Cholecystokinin type A receptor antibody
    see all

Images

  • ICC/IF image of ab77269 stained CaCo2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77269 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab77269 at 10µg/ml staining CCKAR in human pancreas by Immunohistochemistry, Paraffin-embedded tissue (note cytoplasmic staining of some cells in the islets of Langerhans).

References

This product has been referenced in:
  • El-Kouhen K & Morisset J Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line. Pancreas 39:127-34 (2010). ICC/IF . Read more (PubMed: 19959964) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - Triton X-100
Specification
Pancreas
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 19°C
Fixative
Formaldehyde
Username

Ms. Alison Holiday

Verified customer

Submitted Feb 07 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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