Overview

  • Product nameAnti-CCKBR antibody
    See all CCKBR primary antibodies
  • Description
    Rabbit polyclonal to CCKBR
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Cow, Dog
  • Immunogen

    Poly Lysin conjugated synthetic peptide:

    VHRWPSARVRQTWS

    , corresponding to amino acids 206-219 of Human CCKBR.

Properties

Applications

Our Abpromise guarantee covers the use of ab14440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000. Predicted molecular weight: 50 kDa.
IHC-P 1/100.

Target

  • FunctionReceptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
    Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.
  • Tissue specificityIsoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC28 cell lines, not expressed in GLC19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCK B antibody
    • CCK B receptor antibody
    • CCK BR antibody
    • CCK-B receptor antibody
    • CCK-BR antibody
    • CCK2 R antibody
    • CCK2 receptor antibody
    • CCK2-R antibody
    • CCKB antibody
    • CCKB receptor antibody
    • CCKBR antibody
    • CCKR Type B antibody
    • CCKRB antibody
    • Cholecystokinin 2 receptor antibody
    • Cholecystokinin B receptor antibody
    • Cholecystokinin-2 receptor antibody
    • Cholecystokinin2 receptor antibody
    • CholecystokininB receptor antibody
    • GASR antibody
    • GASR_HUMAN antibody
    • Gastrin receptor antibody
    • Gastrin/cholecystokinin type B receptor antibody
    • Gastrin\cholecystokinin brain receptor antibody
    see all

References for Anti-CCKBR antibody (ab14440)

This product has been referenced in:
  • Sellam F  et al. Immunohistochemical examination of cholecystokinin and gastrin receptors (CCK-2/gastrin-R) expression in normal and exocrine cancerous human pancreatic tissues. Pancreatology N/A:N/A (2015). IHC-P, IHC-P ; Human . Read more (PubMed: 26520651) »

See 1 Publication for this product

Product Wall


The pretreatment note in the protocol of the IHC kit refers to the antigen retrieval step that is required for most antibody to achieve a successful IHC-P staining.
For both of these antibodies (ab14440 and ab52894) I can recommend to use Sod...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Guinea pig Tissue sections (hippocampus)
Specification hippocampus
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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Submitted Oct 03 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Chicken Tissue sections (hippocampus)
Specification hippocampus
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 03 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Cat Tissue sections (hippocampus)
Specification hippocampus
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 03 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Rat Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.3% triton
Fixative Methanol
Username

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Verified customer

Submitted Sep 12 2013

Thank you for your enquiry. As this antibody was originated outside of this company, we are unable to provide you with the peptide used as the immunogen. Unfortunately we don't have further information about this antibody. We apologize for the inconven...

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Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"