Overview

  • Product nameAnti-CCKBR antibody
    See all CCKBR primary antibodies
  • Description
    Rabbit polyclonal to CCKBR
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, African Green Monkey
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from the internal region of human CCKBR.

  • Positive control
    • Extracts from COS-7 cells, A549 cells and K562 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab76250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
      Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.
    • Tissue specificityIsoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC28 cell lines, not expressed in GLC19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo.
    • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCK B antibody
      • CCK B receptor antibody
      • CCK BR antibody
      • CCK-B receptor antibody
      • CCK-BR antibody
      • CCK2 R antibody
      • CCK2 receptor antibody
      • CCK2-R antibody
      • CCKB antibody
      • CCKB receptor antibody
      • CCKBR antibody
      • CCKR Type B antibody
      • CCKRB antibody
      • Cholecystokinin 2 receptor antibody
      • Cholecystokinin B receptor antibody
      • Cholecystokinin-2 receptor antibody
      • Cholecystokinin2 receptor antibody
      • CholecystokininB receptor antibody
      • GASR antibody
      • GASR_HUMAN antibody
      • Gastrin receptor antibody
      • Gastrin/cholecystokinin type B receptor antibody
      • Gastrin\cholecystokinin brain receptor antibody
      see all

    Anti-CCKBR antibody images

    • All lanes : Anti-CCKBR antibody (ab76250) at 1/500 dilution

      Lane 1 : Extracts from COS-7 cells
      Lane 2 : Extracts from A549 cells
      Lane 3 : Extracts from K562 cells
      Lane 4 : Extracts from K562 cells plus 5µg immunizing peptide

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 48 kDa
      Observed band size : 48 kDa

    References for Anti-CCKBR antibody (ab76250)

    ab76250 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76250.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"