Overview

  • Product nameAnti-CCKBR antibody
    See all CCKBR primary antibodies
  • Description
    Goat polyclonal to CCKBR
  • Tested applicationsSuitable for: WB, IHC-P, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide:

    C-FDGDSDSDSQSRVRNQ

    , corresponding to amino acids 251-266 of Human CCKBR (NP_795344.1).

  • Positive control
    • Purified membranes of rat insulinoma cell line RIN14B. Human pancreas tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 48 kDa).Can be blocked with CCKBR peptide (ab208978). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022).
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionReceptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
    Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.
  • Tissue specificityIsoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC28 cell lines, not expressed in GLC19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCK B antibody
    • CCK B receptor antibody
    • CCK BR antibody
    • CCK-B receptor antibody
    • CCK-BR antibody
    • CCK2 R antibody
    • CCK2 receptor antibody
    • CCK2-R antibody
    • CCKB antibody
    • CCKB receptor antibody
    • CCKBR antibody
    • CCKR Type B antibody
    • CCKRB antibody
    • Cholecystokinin 2 receptor antibody
    • Cholecystokinin B receptor antibody
    • Cholecystokinin-2 receptor antibody
    • Cholecystokinin2 receptor antibody
    • CholecystokininB receptor antibody
    • GASR antibody
    • GASR_HUMAN antibody
    • Gastrin receptor antibody
    • Gastrin/cholecystokinin type B receptor antibody
    • Gastrin\cholecystokinin brain receptor antibody
    see all

Anti-CCKBR antibody images

  • ab77077 (4µg/ml) staining of paraffin embedded Human Pancreas tissue. Steamed antigen retrieval carried using Tris/EDTA buffer pH 9 followed by HRP-staining.

  • ab77077, at 3µg/ml, staining CCKBR in paraffin-embedded Human pancreas tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Note strong staining in islets of Langerhans and weak staining in acinar cells.

References for Anti-CCKBR antibody (ab77077)

This product has been referenced in:
  • Xu L  et al. Heterobivalent ligands target cell-surface receptor combinations in vivo. Proc Natl Acad Sci U S A : (2012). IHC-Fr . Read more (PubMed: 23236171) »
  • Berna MJ  et al. CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce collagen production. J Biol Chem 285:38905-14 (2010). ICC/IF ; Rat . Read more (PubMed: 20843811) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Sodium
Sample Mouse Tissue sections (brainstem slice)
Specification brainstem slice
Permeabilization Yes - 0.5% triton
Fixative Paraformaldehyde
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Verified customer

Submitted Oct 30 2014

Thank you for contacting us.

I have checked the sequence homology of the immunogen of each antibody witht he sequence of the CCK receptor A.
For ab43404, the homology is 0%, and forab77077, the homology isonly 12.5%. Thus, no cross react...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"