Overview

  • Product name
  • Description
    Rabbit polyclonal to CCL14
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant CCL14 (human Hemofiltrate CC Chemokine 1)

  • General notes


    CCL14 was first isolated from the hemofiltrate of patients with chronic renal failure. It has a 46% amino acid sequence identity with MIP-1a/MIP-1b and 29%-37% homology with other CC chemokines.

Properties

Applications

Our Abpromise guarantee covers the use of ab9845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.

To detect CCL14 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant CCL14.

WB Use a concentration of 0.1 - 0.2 µg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant CCL14 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Function
    Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
  • Tissue specificity
    Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood.
    HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CC-1 antibody
    • CC-3 antibody
    • CCL14 antibody
    • CCL14_HUMAN antibody
    • chemokine (C-C motif) ligand 14 antibody
    • Chemokine CC-1/CC-3 antibody
    • chemokine CC1 antibody
    • chemokine CC3 antibody
    • chemokine HCC1 antibody
    • chemokine HCC3 antibody
    • CKb1 antibody
    • HCC-1(1-74) antibody
    • HCC-1(9-74) antibody
    • HCC-1/HCC-3 antibody
    • HCC-3 antibody
    • HCC1 antibody
    • HEMOFILTRATE CC CHEMOKINE 1 antibody
    • MCIF antibody
    • NCC-2 antibody
    • NCC2 antibody
    • new CC chemokine 2 antibody
    • SCYA14 antibody
    • SCYL2 antibody
    • small inducible cytokine subfamily A (Cys-Cys), member 14 antibody
    • Small-inducible cytokine A14 antibody
    • SY14 antibody
    see all

References

This product has been referenced in:
  • Zhang X  et al. AAV2 delivery of Flt23k intraceptors inhibits murine choroidal neovascularization. Mol Ther 23:226-34 (2015). Read more (PubMed: 25306972) »
  • El-Kasti MM  et al. A novel long-range enhancer regulates postnatal expression of Zeb2: implications for Mowat-Wilson syndrome phenotypes. Hum Mol Genet 21:5429-42 (2012). Read more (PubMed: 23001561) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. First, I'm pleased to hear that ab12326 is working out for you in Wetern blotting. Ab12326 has not yet been tested for application in IP. I do suggest that you submit a review regarding your experiences with the antibody - i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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