Overview

  • Product nameAnti-CCL14 antibody
    See all CCL14 primary antibodies
  • Description
    Rabbit polyclonal to CCL14
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant CCL14 (human Hemofiltrate CC Chemokine 1)

  • General notes


    CCL14 was first isolated from the hemofiltrate of patients with chronic renal failure. It has a 46% amino acid sequence identity with MIP-1a/MIP-1b and 29%-37% homology with other CC chemokines.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.

To detect CCL14 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant CCL14.

WB Use a concentration of 0.1 - 0.2 µg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant CCL14 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • FunctionHas weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
  • Tissue specificityExpressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood.
    HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CC-1 antibody
    • CC-3 antibody
    • CCL14 antibody
    • CCL14_HUMAN antibody
    • chemokine (C-C motif) ligand 14 antibody
    • Chemokine CC-1/CC-3 antibody
    • chemokine CC1 antibody
    • chemokine CC3 antibody
    • chemokine HCC1 antibody
    • chemokine HCC3 antibody
    • CKb1 antibody
    • HCC-1(1-74) antibody
    • HCC-1(9-74) antibody
    • HCC-1/HCC-3 antibody
    • HCC-3 antibody
    • HCC1 antibody
    • HEMOFILTRATE CC CHEMOKINE 1 antibody
    • MCIF antibody
    • NCC-2 antibody
    • NCC2 antibody
    • new CC chemokine 2 antibody
    • SCYA14 antibody
    • SCYL2 antibody
    • small inducible cytokine subfamily A (Cys-Cys), member 14 antibody
    • Small-inducible cytokine A14 antibody
    • SY14 antibody
    see all

References for Anti-CCL14 antibody (ab9845)

ab9845 has not yet been referenced specifically in any publications.

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