Anti-CCL21 antibody [MM0146-2H26] (ab89396)
Key features and details
- Mouse monoclonal [MM0146-2H26] to CCL21
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CCL21 antibody [MM0146-2H26]
See all CCL21 primary antibodies -
Description
Mouse monoclonal [MM0146-2H26] to CCL21 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CCL21.
Database link: O00585 -
Positive control
- WB: Human placenta tissue lysate. IHC-P: Human placenta tissue.
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General notes
Reconstituted antibody should be aliquoted and can be stored frozen at < -20 for at least six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute the antibody with 200 µl sterile PBS to give a final concentration of 0.5 mg/ml. -
Storage instructions
Shipped at 4°C. Store at -80°C. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter. -
Clonality
Monoclonal -
Clone number
MM0146-2H26 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 15 kDa.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 15 kDa. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. -
Tissue specificity
Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6366 Human
- Omim: 602737 Human
- SwissProt: O00585 Human
- Unigene: 57907 Human
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Alternative names
- 6Ckine antibody
- Beta chemokine exodus 2 antibody
- Beta-chemokine exodus-2 antibody
see all
Images
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Anti-CCL21 antibody [MM0146-2H26] (ab89396) at 1/500 dilution + Human placenta tissue lysate
Predicted band size: 15 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CCL21 with ab89396. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab89396 has not yet been referenced specifically in any publications.