The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 14 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant human CCL28 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 0.25 - 1 µg/ml. Can be paired for ELISA with Rabbit polyclonal to CCL28 (ab113648). May be used in direct ELISA; allows the detection of at least 0.2 to 0.4 ng/well of recombinant human CCL28.
Use a concentration of 0.25 - 1 µg/ml. May be used in conjunction with ab113648 as a capture antibody, allows the detection of at least 0.2 to 0.4 ng/well of recombinant human CCL28.
FunctionChemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.
Tissue specificityPreferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.