Recombinant
RabMAb

Anti-CCL6 [EPR14614] antibody (ab191400)

Overview

  • Product name
    Anti-CCL6 [EPR14614] antibody
    See all CCL6 primary antibodies
  • Description
    Rabbit monoclonal [EPR14614] to CCL6
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse CCL6 aa 1-100. The exact sequence is proprietary.
    Database link: P27784

  • Positive control
    • Recombinant mouse CCL6 protein, mouse and rat spleen tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14614
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance
    CCL6 (C10) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It is a potent chemoattractant of macrophages, but it can also attract B cells, CD4+ lymphocytes and eosinophils. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be induced under conditions suitable for myeloid cell differentiation. CCL6 (C10) was originally identified as a transcript that is induced in bone marrow cells upon stimulation of GMCSF. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • C10 antibody
    • CCL 6 antibody
    • chemokine (C-C motif) ligand 6 antibody
    • Chemokine C C motif Ligand 6 antibody
    • MRP1 antibody
    • SCYA6 antibody
    • Small Inducible Cytokine A6 antibody
    see all

Images

  • Anti-CCL6 [EPR14614] antibody (ab191400) at 1/10000 dilution + Recombinant mouse CCL6 protein at 20 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 13 kDa
    Observed band size : 13 kDa
  • Immunohistochemical analysis of paraffin-embedded, mouse spleen tissue labeling CCL6 with ab191400 at a 1/2000 dilution (0.6μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

  • Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling CCL6 with ab191400 at a 1/2000 dilution (0.6μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

References

ab191400 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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