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Recombinant fragment: IKDPMEDQDG LLGKELGFDA SEVELTRNNE LIAVGKGSSL KRLPVFGMEP RILYNPLQGQ KCIVQTTSWS QCSKT, corresponding to amino acids 168-242 of human CCN1 purified from E. coli
ab80112 is derived from hybridization of mouse FO myeloma cells with spleen cells from immunized BALB/c mice.
The isotype is mouse IgG1 heavy chain and kappa light chain.
Our Abpromise guarantee covers the use of ab80112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/250 - 1/1000. Predicted molecular weight: 42 kDa.|
|ELISA||Use at an assay dependent concentration.|
ab80112 at 1/50 dilution staining CCN1 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) staining technique.