• Product name
    Anti-CCN1 antibody [3H3]
    See all CCN1 primary antibodies
  • Description
    Mouse monoclonal [3H3] to CCN1
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment: IKDPMEDQDG LLGKELGFDA SEVELTRNNE LIAVGKGSSL KRLPVFGMEP RILYNPLQGQ KCIVQTTSWS QCSKT, corresponding to amino acids 168-242 of human CCN1 purified from E. coli

  • Positive control
    • Jurkat cell lysates.
  • General notes

    ab80112 is derived from hybridization of mouse FO myeloma cells with spleen cells from immunized BALB/c mice.
    The isotype is mouse IgG1 heavy chain and kappa light chain.



Our Abpromise guarantee covers the use of ab80112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/50.
WB 1/250 - 1/1000. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent concentration.


  • Function
    Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
  • Sequence similarities
    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • 3CH61 antibody
    • CCN 1 antibody
    • CCN family member 1 antibody
    • CYR 61 antibody
    • Cyr61 antibody
    • CYR61 protein antibody
    • CYR61_HUMAN antibody
    • Cysteine rich angiogenic inducer 61 antibody
    • Cysteine rich heparin binding protein 61 antibody
    • Cysteine-rich angiogenic inducer 61 antibody
    • GIG 1 antibody
    • GIG1 antibody
    • IBP-10 antibody
    • IGF-binding protein 10 antibody
    • Igfbp 10 antibody
    • IGFBP-10 antibody
    • Igfbp10 antibody
    • Insulin like growth factor binding protein 10 antibody
    • Insulin-like growth factor-binding protein 10 antibody
    • Protein CYR61 antibody
    • Protein GIG1 antibody
    see all

Anti-CCN1 antibody [3H3] images

  • Anti-CCN1 antibody [3H3] (ab80112) at 1/250 dilution + Jurkat cell lysate at 40 µg

    goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab80112 at 1/50 dilution staining CCN1 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) staining technique.

  • ICC/IF image of ab80112 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80112, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CCN1 antibody [3H3] (ab80112)

This product has been referenced in:
  • Cardoso J  et al. CYR61 and TAZ Upregulation and Focal Epithelial to Mesenchymal Transition May Be Early Predictors of Barrett's Esophagus Malignant Progression. PLoS One 11:e0161967 (2016). IHC-P ; Human . Read more (PubMed: 27583562) »
  • Ma L  et al. Fat4 suppression induces Yap translocation accounting for the promoted proliferation and migration of gastric cancer cells. Cancer Biol Ther 17:36-47 (2016). WB ; Human . Read more (PubMed: 26575609) »

See all 2 Publications for this product

Product Wall

Immuno-precipitation step
Protein A/G
Mouse Cell lysate - whole cell (osteosarcoma)
Total protein in input
200 µg

Abcam user community

Verified customer

Submitted Jul 15 2014

Immuno-precipitation step
Protein A/G
Human Cell lysate - whole cell (osteosarcoma)
Total protein in input
200 µg

Abcam user community

Verified customer

Submitted Jun 26 2014

Thank you for contacting us.

I confirm the sodium citrate concentration used was 0.1M.

Both 0.1M and 0.01M are fine. In our online protocols we specify using 0.01M concentration for the buffer, but if 0.1M concentration is used inst...

Read More


Sign up