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Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human CCN1.
(Peptide available as ab24416.)
Our Abpromise guarantee covers the use of ab10760 in the following tested applications.
ab10760 staining CCN1 in mouse kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded). Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in Tris-EDTA buffer pH9.0 and bloking for 15 minutes at 20°C (5 minutes/peroxidase block and 10 minutes/protein block). The primary antibody was diluted 1/100 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used, undiluted as the secondary.
ab10760 has not yet been referenced specifically in any publications.