This antibody gave a positive signal in the following lysates:
Hela nuclear cell extract
Hela cell lysate.
A431 cell lysate.
Jurkat cell lysate
Liver (Mouse) tissue lysate
Heart (Mouse) tissue lysate
Kidney (Mouse) tissue lysate
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a dilution of 1/100 for 1 hr.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 42-50 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Insulin like growth factor binding protein 10 antibody
Insulin-like growth factor-binding protein 10 antibody
Protein CYR61 antibody
Protein GIG1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CCN1 antibody (ab10760)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
ab10760 staining CCN1 in mouse kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded). Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in Tris-EDTA buffer pH9.0 and bloking for 15 minutes at 20°C (5 minutes/peroxidase block and 10 minutes/protein block). The primary antibody was diluted 1/100 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used, undiluted as the secondary.