Overview

  • Product name
  • Description
    Rabbit polyclonal to CCNL1
  • Specificity
    This antibody is specific for human Cyclin L1a and shows negligible reactivity to Cyclin L1 beta or Cyclin L2 isoforms.
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide: KLKAKGL NP DGTPALST L GGFSPASKP SSPREVKAEE KSPISINVK Q, corresponding to amino acids 314-369 of Human CCNL1

  • Positive control
    • For Western blotting : Mouse brain whole cell lysate. For IHC: A variety of tissues including breast, heart, kidney [distal tubules], liver, skeletal muscle, spleen.

Properties

Applications

Our Abpromise guarantee covers the use of ab34709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IHC-P
IP
ICC/IF
  • Application notes
    ELISA: 1/5000 - 1/25,000.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 10 - 20 µg/ml.
    IP: Use at an assay dependent dilution.
    WB: 1/2000 - 1/5000. Predicted molecular weight: 59 kDa. Approximately 35µg of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:3,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcriptional regulator which participates in regulating the pre-mRNA splicing process. Seems to be involved in the regulation of RNA polymerase II (pol II). Functions in association with cyclin-dependent kinases (CDKs) and has a role in the second step of splicing. May be a candidate proto-oncogene in head and neck squamous cell carcinomas (HNSCC). Inhibited by the CDK-specific inhibitor p21.
    • Tissue specificity
      Ubiquitous with higher level in thymus. Overexpression in primary tumors of head and neck squamous cell carcinomas (HNSCC).
    • Sequence similarities
      Belongs to the cyclin family. Cyclin L subfamily.
    • Domain
      Contains a RS region (arginine-serine dipeptide repeat) within the C-terminal domain which is the hallmark of the SR family of splicing factors. This region probably plays a role in protein-protein interactions.
    • Cellular localization
      Nucleus speckle. More specifically found in nuclear intrachromatin granules clusters (IGC), also called nuclear speckles, which are storage compartments for nuclear proteins involved in mRNA processing.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ania 6a antibody
      • CCNL1 antibody
      • CCNL1_HUMAN antibody
      • Cyclin L antibody
      • Cyclin L gamma antibody
      • Cyclin L1 antibody
      • Cyclin-L antibody
      • Cyclin-L1 antibody
      see all

    Images

    • This image shows human spleen stained with ab34709 at 10µg/ml

    References

    ab34709 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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