Overview

  • Product nameAnti-CCNL1 antibody
    See all CCNL1 primary antibodies
  • Description
    Mouse monoclonal to CCNL1
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human CCNL1 protein (AAH38394, 1 a.a. ~ 173 a.a), with a tag.

  • Positive control
    • CCNL1 transfected 293T cell lysate. Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab68353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
ELISA
  • Application notesELISA: Use at an assay dependent concentration. Detection limit for recombinant tagged CCNL1 is approximately 3ng/ml as a capture antibody.
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: Isoform 5 (Cyclin L gamma) 18kDa; Longest Isoform (Cyclin L alpha) 60 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional regulator which participates in regulating the pre-mRNA splicing process. Seems to be involved in the regulation of RNA polymerase II (pol II). Functions in association with cyclin-dependent kinases (CDKs) and has a role in the second step of splicing. May be a candidate proto-oncogene in head and neck squamous cell carcinomas (HNSCC). Inhibited by the CDK-specific inhibitor p21.
    • Tissue specificityUbiquitous with higher level in thymus. Overexpression in primary tumors of head and neck squamous cell carcinomas (HNSCC).
    • Sequence similaritiesBelongs to the cyclin family. Cyclin L subfamily.
    • DomainContains a RS region (arginine-serine dipeptide repeat) within the C-terminal domain which is the hallmark of the SR family of splicing factors. This region probably plays a role in protein-protein interactions.
    • Cellular localizationNucleus speckle. More specifically found in nuclear intrachromatin granules clusters (IGC), also called nuclear speckles, which are storage compartments for nuclear proteins involved in mRNA processing.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ania 6a antibody
      • CCNL1 antibody
      • CCNL1_HUMAN antibody
      • Cyclin L antibody
      • Cyclin L gamma antibody
      • Cyclin L1 antibody
      • Cyclin-L antibody
      • Cyclin-L1 antibody
      see all

    Anti-CCNL1 antibody images

    • All lanes : Anti-CCNL1 antibody (ab68353) at 1 µg/ml

      Lane 1 : CCNL1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
    • Immunofluorescent staining of CCNL1 in HeLa cells using ab68353 at 10µg/ml.

    References for Anti-CCNL1 antibody (ab68353)

    ab68353 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"