The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Used with acetone fixative on Rheumatoid synovium and tonsil. Detects endogenous protein, also used with full length CCR3 expressed from a CMV based promoter in L1.2 cells.
Use at an assay dependent concentration. ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionReceptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
Tissue specificityIn eosinophils as well as trace amounts in neutrophils and monocytes.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.