The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells.
ab91534 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
In eosinophils as well as trace amounts in neutrophils and monocytes.
Belongs to the G-protein coupled receptor 1 family.
Flow cytometric analysis of normal Human peripheral blood cells labeling CCR3 with Anti-Human CD16 PE and mouse IgG2b kappa Isotype Control APC (left) or 5 µl (0.25 µg) of ab155356 (right). Cells in the large scatter population were used for analysis.