Overview

  • Product nameAnti-CCR3 antibody
    See all CCR3 primary antibodies
  • Description
    Goat polyclonal to CCR3
  • SpecificityPeptide sequence is < 50 % identical to other human chemokine receptors in this region.
  • Tested applicationsSuitable for: ICC, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: C-VETFGTTSYYDDVGLLCEKADTRALMA, corresponding to N terminal amino acids 8-34 of Human CCR3.

  • Positive control
    • This anitbody gave a positive signal in human lung, ovary and thymus tissue lysates.
  • General notes


    Human CCR 3 is a receptor for CC chemokine eotaxin-1 and eotaxin-2. CCR 3 is constitutively expressed on eosinophils, small intestine and colon. The human CCR 3 protein contains 356 amino acids.

Properties

Applications

Our Abpromise guarantee covers the use of ab1667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/150.
IHC-P 1/150.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

  • FunctionReceptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Tissue specificityIn eosinophils as well as trace amounts in neutrophils and monocytes.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B chemokine receptor antibody
    • b-chemokine receptor antibody
    • C C chemokine receptor type 3 antibody
    • C C CKR 3 antibody
    • C-C chemokine receptor type 3 antibody
    • C-C CKR-3 antibody
    • CC chemokine receptor 3 antibody
    • CC chemokine receptor type 3 antibody
    • CC CKR 3 antibody
    • CC R3 antibody
    • CC-CKR-3 antibody
    • CCCKR3 antibody
    • CCR 3 antibody
    • CCR-3 antibody
    • CCR3 antibody
    • CCR3_HUMAN antibody
    • CD 193 antibody
    • CD193 antibody
    • CD193 antigen antibody
    • Chemokine (C C motif) receptor 3 antibody
    • chemokine (C-C motif) receptor 3 antibody
    • Chemokine (CC) receptor 1 like 2 antibody
    • Chemokine CC motif receptor 3 antibody
    • Chemokine receptor 3 antibody
    • CK R3 antibody
    • CKR 3 antibody
    • CKR3 antibody
    • CMKB R3 antibody
    • CMKBR 3 antibody
    • CMKBR1L2 antibody
    • CMKBR3 antibody
    • Eosinophil CC chemokine receptor 3 antibody
    • Eosinophil eotaxin receptor antibody
    • Eotaxin receptor antibody
    • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
    • MGC102841 antibody
    • MIP1 Alpha RL2 antibody
    see all

Anti-CCR3 antibody images

  • Immunocytochemistry on human peripheral blood cells (acetone fixed cells).
  • All lanes : Anti-CCR3 antibody (ab1667) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)
    Lane 3 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-CCR3 antibody (ab1667)

This product has been referenced in:
  • Jöhrer K  et al. Up-regulation of functional chemokine receptor CCR3 in human renal cell carcinoma. Clin Cancer Res 11:2459-65 (2005). Read more (PubMed: 15814620) »

See 1 Publication for this product

Product Wall

Thank you for your reply. A quotation has been sent to you in a separate email, the proforma reference number is : *****. DISCOUNT CODE for ab1667 to be tested in Flow Cytometry : ******** Expiration date: DD MM YYYY I am very pleased to hear you would...

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Thank you very much for your interest in our anti-CCR3 antibodies. To our knowledge, none of ouranti-CCR3 antibodieshas not been tested in Flow Cytometry as well as in IHC-P. Therefore, I can offer a discount off a future purchase if you buy an anti-CC...

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Thank you for your enquiry. Ab1667: Antigen Retrieval: Sections were treated with antigen retrieval solution (Dako # S-1700, pH 6.0) and steamed for 20 minutes followed by cooling for 20 minutes. Variations of the method will work successfully as...

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