Recombinant
RabMAb

Anti-CCR3 antibody [Y31] (ab32512)

Overview

  • Product name
    Anti-CCR3 antibody [Y31]
    See all CCR3 primary antibodies
  • Description
    Rabbit monoclonal [Y31] to CCR3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Macaque monkey
  • Immunogen

    A synthetic peptide corresponding to residues in C-terminal cytoplasmic domain of human CKR3.

  • Positive control
    • K562 and Daudi cell lysates and human liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-CCR3 antibody (HRP) [Y31] (ab200020)

Properties

Applications

Our Abpromise guarantee covers the use of ab32512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 43 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
    • Tissue specificity
      In eosinophils as well as trace amounts in neutrophils and monocytes.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • B chemokine receptor antibody
      • b-chemokine receptor antibody
      • C C chemokine receptor type 3 antibody
      • C C CKR 3 antibody
      • C-C chemokine receptor type 3 antibody
      • C-C CKR-3 antibody
      • CC chemokine receptor 3 antibody
      • CC chemokine receptor type 3 antibody
      • CC CKR 3 antibody
      • CC R3 antibody
      • CC-CKR-3 antibody
      • CCCKR3 antibody
      • CCR 3 antibody
      • CCR-3 antibody
      • CCR3 antibody
      • CCR3_HUMAN antibody
      • CD 193 antibody
      • CD193 antibody
      • CD193 antigen antibody
      • Chemokine (C C motif) receptor 3 antibody
      • chemokine (C-C motif) receptor 3 antibody
      • Chemokine (CC) receptor 1 like 2 antibody
      • Chemokine CC motif receptor 3 antibody
      • Chemokine receptor 3 antibody
      • CK R3 antibody
      • CKR 3 antibody
      • CKR3 antibody
      • CMKB R3 antibody
      • CMKBR 3 antibody
      • CMKBR1L2 antibody
      • CMKBR3 antibody
      • Eosinophil CC chemokine receptor 3 antibody
      • Eosinophil eotaxin receptor antibody
      • Eotaxin receptor antibody
      • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
      • MGC102841 antibody
      • MIP1 Alpha RL2 antibody
      see all

    Anti-CCR3 antibody [Y31] images

    • Immunohistochemical analysis of paraffin-embedded human liver tissue using ab32512 at 1/100 dilution.
    • All lanes : Anti-CCR3 antibody [Y31] (ab32512) at 1/500 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Daudi cell lysate


      Predicted band size : 43 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    References for Anti-CCR3 antibody [Y31] (ab32512)

    This product has been referenced in:
    • Kuse Y  et al. CCR3 Is Associated with the Death of a Photoreceptor Cell-line Induced by Light Exposure. Front Pharmacol 8:207 (2017). WB ; Mouse . Read more (PubMed: 28458639) »
    • Sun RL  et al. CC-Chemokine Ligand 2 (CCL2) Suppresses High Density Lipoprotein (HDL) Internalization and Cholesterol Efflux via CC-Chemokine Receptor 2 (CCR2) Induction and p42/44 Mitogen-activated Protein Kinase (MAPK) Activation in Human Endothelial Cells. J Biol Chem 291:19532-44 (2016). WB ; Human . Read more (PubMed: 27458015) »

    See all 9 Publications for this product

    Product Wall

    Thank you very much for your reply and for sending this additional information.

    Your credit note ID is ***.

    I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the origina...

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    Thank you very much for contacting us and letting us know about the trouble with these antibodies.

    Thank you also for sending so many details of your protocol.The protocol seems to be ok and similar to the protocol that the lab uses to test t...

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    Thank you for your inquiry.

    In general we recommend to use EDTA pH9. This antigen retrieval method is optimal for the most antigens. Please see the attachment and this publication (Emoto et al., J Histochem Cytochem. 2005 Nov ;53 (11) : 1311-...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Loading amount
    100000 cells
    Specification
    Jurkat cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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