Anti-CCR4 antibody [KH-4F5] (ab59550)
Key features and details
- Mouse monoclonal [KH-4F5] to CCR4
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-CCR4 antibody [KH-4F5]
See all CCR4 primary antibodies -
Description
Mouse monoclonal [KH-4F5] to CCR4 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide from human CCR4
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KH-4F5 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1.2µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use 1µg for 106 cells.
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Notes |
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Flow Cyt
Use 1.2µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use 1µg for 106 cells. |
Target
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Function
High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. -
Tissue specificity
Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsIn natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- C C chemokine receptor type 4 antibody
- C C CKR 4 antibody
- C-C chemokine receptor type 4 antibody
see all
Images
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Spectral Confocal Microscopy of CHO cells using ab59550 at 1 µg/106 cells . CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of ab59550 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
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FACS analysis of BOSC23 cells using ab59550 at 1.2 µg/ 106 cells. BOSC23 cells were transiently transfected with an expression vector encodingeither CCR4 (red curve) or an irrelevant protein(control transfectant). Binding of ab59550 was detected with a PE conjugated secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab59550 has been referenced in 1 publication.
- Tian Q et al. The regulatory roles of T helper cells in distinct extracellular matrix characterization in breast cancer. Front Immunol 13:871742 (2022). PubMed: 36159822