Anti-CCR4 NOT transcription complex subunit 3 antibody (ab55681)


  • Product nameAnti-CCR4 NOT transcription complex subunit 3 antibody
    See all CNOT3 primary antibodies
  • Description
    Mouse monoclonal to CCR4 NOT transcription complex subunit 3
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human CNOT3 aa 1-101.




Our Abpromise guarantee covers the use of ab55681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.


  • FunctionThe CCR4-NOT complex functions as general transcription regulation complex.
  • Tissue specificityUbiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the CNOT2/3/5 family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCR4 associated factor 3 antibody
    • CCR4-associated factor 3 antibody
    • CCR4-NOT transcription complex subunit 3 antibody
    • CNOT3 antibody
    • CNOT3_HUMAN antibody
    • KIAA0691 antibody
    • LENG2 antibody
    • Leukocyte receptor cluster member 2 antibody
    • negative regulator of transcription 3, S. cerevisiae, homolog of antibody
    • NOT3 (negative regulator of transcription 3, yeast) homolog antibody
    • NOT3 antibody
    • NOT3H antibody
    see all

Anti-CCR4 NOT transcription complex subunit 3 antibody images

  • Predicted band size : 82 kDa
    CCR4 NOT transcription complex subunit 3 antibody (ab55681) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55681 at 10 ug/ml staining CCR4 NOT transcription complex subunit 3 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing Jurkat cells stained with ab55681 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55681, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-CCR4 NOT transcription complex subunit 3 antibody (ab55681)

This product has been referenced in:
  • Neely GG  et al. A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Cell 141:142-53 (2010). ICC/IF ; Mouse . Read more (PubMed: 20371351) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (ES cells)
Loading amount 200000 cells
Specification ES cells
Gel Running Conditions Non-reduced Denaturing (NuPage 4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Jan 16 2013