Anti-CCR4 NOT transcription complex subunit 3 antibody (ab55681)

Overview

  • Product name
    Anti-CCR4 NOT transcription complex subunit 3 antibody
    See all CNOT3 primary antibodies
  • Description
    Mouse monoclonal to CCR4 NOT transcription complex subunit 3
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human CNOT3 aa 1-101.
    Sequence:

    MADKRKLQGEIDRCLKKVSEGVEQFEDIWQKLHNAANANQKEKYEADLKK EIKKLQRLRDQIKTWVASNEIKDKRQLIDNRKLIETQMERFKVVERETKT

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab55681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    The CCR4-NOT complex functions as general transcription regulation complex.
  • Tissue specificity
    Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the CNOT2/3/5 family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCR4 associated factor 3 antibody
    • CCR4-associated factor 3 antibody
    • CCR4-NOT transcription complex subunit 3 antibody
    • CNOT3 antibody
    • CNOT3_HUMAN antibody
    • KIAA0691 antibody
    • LENG2 antibody
    • Leukocyte receptor cluster member 2 antibody
    • negative regulator of transcription 3, S. cerevisiae, homolog of antibody
    • NOT3 (negative regulator of transcription 3, yeast) homolog antibody
    • NOT3 antibody
    • NOT3H antibody
    see all

Anti-CCR4 NOT transcription complex subunit 3 antibody images



  • Predicted band size : 82 kDa
    CCR4 NOT transcription complex subunit 3 antibody (ab55681) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55681 at 10 ug/ml staining CCR4 NOT transcription complex subunit 3 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing Jurkat cells stained with ab55681 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55681, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-CCR4 NOT transcription complex subunit 3 antibody (ab55681)

This product has been referenced in:
  • Neely GG  et al. A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Cell 141:142-53 (2010). ICC/IF ; Mouse . Read more (PubMed: 20371351) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (ES cells)
Loading amount
200000 cells
Specification
ES cells
Gel Running Conditions
Non-reduced Denaturing (NuPage 4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Jan 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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