Anti-CCR5 antibody [mAbcam 64620] (ab64620)

Overview

  • Product nameAnti-CCR5 antibody [mAbcam 64620]
    See all CCR5 primary antibodies
  • Description
    Mouse monoclonal [mAbcam 64620] to CCR5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Baboon, Macaque Monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CCR5.

  • Positive control
    • This antibody gave a positive signal in human spleen tissue lysate and in the following whole cell lysates: THP1; Jurkat; K562.

Properties

Applications

Our Abpromise guarantee covers the use of ab64620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

  • FunctionReceptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
  • Tissue specificityHighly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
  • Involvement in diseaseGenetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
    O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
    Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
    Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AM4 7 antibody
    • C C chemokine receptor type 5 antibody
    • C C CKR 5 antibody
    • C-C chemokine receptor type 5 antibody
    • C-C CKR-5 antibody
    • C-C motif chemokine receptor 5 A159A antibody
    • CC Chemokine Receptor 5 antibody
    • CC Chemokine Receptor Type 5 antibody
    • CC CKR 5 antibody
    • CC-CKR-5 antibody
    • CCCKR 5 antibody
    • CCCKR5 antibody
    • CCR 5 antibody
    • CCR-5 antibody
    • CCR5 antibody
    • CCR5 chemokine (C C motif) receptor 5 antibody
    • CCR5_HUMAN antibody
    • CD 195 antibody
    • CD195 antibody
    • CD195 Antigen antibody
    • Chemokine C C motif receptor 5 antibody
    • Chemokine receptor CCR5 antibody
    • CHEMR13 antibody
    • CKR 5 antibody
    • CKR5 antibody
    • CMKBR 5 antibody
    • CMKBR5 antibody
    • FLJ78003 antibody
    • HIV 1 Fusion Coreceptor antibody
    • HIV-1 fusion coreceptor antibody
    • HIV1 fusion coreceptor antibody
    • IDDM22 antibody
    • MIP-1 alpha receptor antibody
    see all

Anti-CCR5 antibody [mAbcam 64620] images

  • All lanes : Anti-CCR5 antibody [mAbcam 64620] (ab64620) at 10 µg/ml

    Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 4 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 51 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References for Anti-CCR5 antibody [mAbcam 64620] (ab64620)

ab64620 has not yet been referenced specifically in any publications.

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These peptides are very short fragments of the CCR5 protein and as such may not contain the gp120 binding site. You may wish to use the whole protein. While this is not currently available at Abcam...

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