Overview

  • Product name
    Anti-CCR6 antibody [4C6]
    See all CCR6 primary antibodies
  • Description
    Mouse monoclonal [4C6] to CCR6
  • Tested applications
    Suitable for: Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MSGESMNFSD VFDSSEDYFV SVNTSYYSVD SEMLLCSLQE VRQFSR, corresponding to amino acids 1-46 of Human CCR6, purified from E. coli

  • Positive control
    • Cell lysates of Raji

Properties

Applications

Our Abpromise guarantee covers the use of ab68131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ELISA Use at an assay dependent concentration.

Target

  • Function
    Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level.
  • Tissue specificity
    Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BN 1 antibody
    • BN1 antibody
    • C C chemokine receptor type 6 antibody
    • C C CKR 6 antibody
    • C-C chemokine receptor type 6 antibody
    • C-C CKR-6 antibody
    • CC chemokine receptor type 6 antibody
    • CC CKR 6 antibody
    • CC R6 antibody
    • CC-CKR-6 antibody
    • CCCKR6 antibody
    • CCR 6 antibody
    • CCR-6 antibody
    • Ccr6 antibody
    • CCR6_HUMAN antibody
    • CD 196 antibody
    • CD196 antibody
    • CD196 antigen antibody
    • Chemokine (C C motif) receptor 6 antibody
    • Chemokine (C C) receptor 6 antibody
    • Chemokine (CC motif) receptor 6 antibody
    • Chemokine (CC) receptor 6 antibody
    • Chemokine receptor 6 antibody
    • Chemokine receptor like 3 antibody
    • Chemokine receptor-like 3 antibody
    • CKR 6 antibody
    • CKR L3 antibody
    • CKR-L3 antibody
    • CKR6 antibody
    • CKRL3 antibody
    • CMKBR 6 antibody
    • CMKBR6 antibody
    • DCR 2 antibody
    • DCR2 antibody
    • DRY 6 antibody
    • DRY6 antibody
    • G protein coupled receptor 29 antibody
    • G-protein coupled receptor 29 antibody
    • GPR 29 antibody
    • GPR CY4 antibody
    • GPR-CY4 antibody
    • GPR29 antibody
    • GPRCY 4 antibody
    • GPRCY4 antibody
    • LARC receptor antibody
    • Seven transmembrane receptor lymphocyte 22 antibody
    • STRL 22 antibody
    • STRL22 antibody
    see all

Images

  • Anti-CCR6 antibody [4C6] (ab68131) at 1/500 dilution + cell lysates of Raji at 30 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • Overlay histogram showing Jurkat cells stained with ab68131 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68131, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

ab68131 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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