The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionReceptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
Tissue specificityExpressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Flow cytometry staining of normal Human peripheral blood cells with Anti-Human CD45RA FITC and 1.0 µg of Rat IgG2a ? Isotype Control Biotin (left) or 1.0 µg of ab95666 (right) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
References for Anti-CCR7 antibody [3D12] (Biotin) (ab95666)
This product has been referenced in:
Geginat J et al. Proliferation and differentiation potential of human CD8+ memory T-cell subsets in response to antigen or homeostatic cytokines. Blood101:4260-6 (2003).
Read more (PubMed: 12576317) »
Sallusto F et al. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature401:708-12 (1999).
Read more (PubMed: 10537110) »