The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells in a 37°C waterbath for 30 mins.
Note: If red blood cell lysis is carried out as part of cell preparation, ensure that fixatives are not present in the cell lysis solution, as this will eliminate ab93519 staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
Belongs to the G-protein coupled receptor 1 family.
References for Anti-CCR7 antibody [4B12] (Biotin) (ab93519)
This product has been referenced in:
Waldner H et al. The autoimmune diabetes locus Idd9 regulates development of type 1 diabetes by affecting the homing of islet-specific T cells. J Immunol176:5455-62 (2006).
Read more (PubMed: 16622013) »
Ohl L et al. CCR7 governs skin dendritic cell migration under inflammatory and steady-state conditions. Immunity21:279-88 (2004).
Read more (PubMed: 15308107) »