Overview

  • Product nameAnti-CCR7 antibody
    See all CCR7 primary antibodies
  • Description
    Goat polyclonal to CCR7
  • SpecificityPeptide sequence is < 50 % identical to other mouse chemokine receptors in this region. The antibody has been tested in an academic lab on cells transfected with CCR7 receptor, we do not have information regarding its use against endogenous protein.
  • Tested applicationsSuitable for: ELISA, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    DPGKPRKNVLVVALLVIFQVC

    , corresponding to amino acids 2-22 of Mouse CCR7.

  • Positive control
    • IHC-P: Mouse spleen, human spleen. WB: cells transfected with CCR7 receptor

Properties

Applications

Our Abpromise guarantee covers the use of ab1657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100000.
Flow Cyt 1/10.
IHC-P 1/250.
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionReceptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
  • Tissue specificityExpressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BLR 2 antibody
    • BLR2 antibody
    • C C chemokine receptor type 7 antibody
    • C C CKR 7 antibody
    • C-C chemokine receptor type 7 antibody
    • C-C CKR-7 antibody
    • CC chemokine receptor 7 antibody
    • CC chemokine receptor type 7 antibody
    • CC CKR 7 antibody
    • CC-CKR-7 antibody
    • CCCKR7 antibody
    • CCR 7 antibody
    • CCR-7 antibody
    • Ccr7 antibody
    • CCR7_HUMAN antibody
    • CD 197 antibody
    • CD197 antibody
    • CD197 antigen antibody
    • CDw197 antibody
    • Chemokine (C C motif) receptor 7 antibody
    • Chemokine (C C) receptor 7 antibody
    • Chemokine C C motif receptor 7 antibody
    • Chemokine C C receptor 7 antibody
    • Chemokine receptor 7 antibody
    • Chemokine receptor 7-like protein antibody
    • CMKBR7 antibody
    • EBI 1 antibody
    • EBI1 antibody
    • Ebi1h antibody
    • EBV Induced G Protein Coupled Receptor 1 antibody
    • EBV-induced G-protein coupled receptor 1 antibody
    • Epstein Barr virus induced G protein coupled receptor antibody
    • Epstein Barr virus induced gene 1 antibody
    • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
    • EVI 1 antibody
    • EVI1 antibody
    • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
    • MGC108519 antibody
    • MIP 3 beta receptor antibody
    • MIP-3 beta receptor antibody
    • MIP3 Beta Receptor antibody
    see all

Anti-CCR7 antibody images

  • Immunohistochemical staining for CCR7 in human spleen.

  • ab1657, at a dilution of 1/250, staining CCR7 in mouse spleen section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Anti-CCR7 antibody (ab1657) at 1 µg/ml + K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa


    Exposure time : 20 minutes

References for Anti-CCR7 antibody (ab1657)

This product has been referenced in:
  • Salim SY  et al. CD83+CCR7- dendritic cells accumulate in the subepithelial dome and internalize translocated Escherichia coli HB101 in the Peyer's patches of ileal Crohn's disease. Am J Pathol 174:82-90 (2009). IHC-P ; Human . Read more (PubMed: 19095953) »
  • Pitkin L  et al. Expression of CC chemokine receptor 7 in tonsillar cancer predicts cervical nodal metastasis, systemic relapse and survival. Br J Cancer 97:670-7 (2007). IHC-P ; Human . Read more (PubMed: 17687340) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Sample Mouse Tissue sections (spleen)
Specification spleen
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 19 2014

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spleen, lymph node)
Specification spleen, lymph node
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Jong Hyung Lim

Verified customer

Submitted Nov 12 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step methanol/hydrogen peroxide (and I also tried 10% BSA) as blocking agent for 5 minute(s) · Concentration: 2.5%
Username

Abcam user community

Verified customer

Submitted May 30 2007

Thank you for your patience. I have been in touch with the source of this antibody once again and I have some additional information. Unfortunately at this time I cannot source an alternative lot. However, the originator mentioned the following val...

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Thank you for taking the time to complete our questionaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody (ab16527). I have checked the procedure used to test this antibody in the laborat...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10%
Username

Dr. Linda Svantesson

Verified customer

Submitted Nov 17 2006

Thank you for your enquiry. I am sorry to hear that you have been obtaining non-specific staining using this antibody. I have read your technical questionnaire and I have a few comments. Please can you detail the duration of antigen retrieval t...

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Thank you for your enquiry. Further to our telephone conversation yesterday afternoon I have been in touch with the source of this antibody. They informed me that by western blotting an actual molecular weight of 43KDa was detected in the following...

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Thank you for your e-mail. We hope these answers are more helpful. 5. How did you block the unspecific binding sites? BSA 3% 30 minutes Instead, use 2% normal rabbit serum at room temperature to block the non-specific binding sites. 7. Seco...

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We are very sorry you are having problems with your protocol. Here is the up to date detailed protocol: .

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"