Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CCR7 (N terminal).
Database link: P32248
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab32527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).Can be blocked with CCR7 peptide (ab209386). For unpurified use at 1/5000. |
|
ICC/IF | 1/100. For unpurified use at 1/250. |
|
IP | 1/20. For unpurified use at 1/10. |
Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling CCR7 with purified ab32527 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CCR7 with unpurified ab32527 at 1/1000. Cells were fixed with 4% PFA fixed (10 min) and permeabilized and blocked with 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody (ab32527, 1µg/ml) overnight at +4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (shown in green). Alexa Fluor® 594 WGA was used to label plasma membranes (shown in red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
ab32527 (purified) at 1/20 immunoprecipitating CCR7 in MCF-7 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/10,000) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"