Anti-CCR7 antibody [Y59] (ab32527)
- Datasheet
- Specific References (25)
- Protocols
Overview
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Product nameAnti-CCR7 antibody [Y59]
See all CCR7 primary antibodies -
DescriptionRabbit monoclonal [Y59] to CCR7
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Host speciesRabbit
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SpecificityThe antibody does not cross-react with other G-protein coupled receptor 1 family members.
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Tested applicationsSuitable for: WB, ICC/IF, IPmore details
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Species reactivityReacts with: Mouse, Rat, Human, Rhesus monkey
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CCR7 (N terminal).
Database link: P32248 -
Positive control
- WB: K562 and Daudi cell lysates. ICC/IF: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberY59
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
- Anti-CCR7 antibody [Y59] (Alexa Fluor® 488) (ab196459)
- Anti-CCR7 antibody [Y59] (HRP) (ab196475)
- Anti-CCR7 antibody [Y59] (Alexa Fluor® 647) (ab196640)
- Anti-CCR7 antibody [Y59] (Alexa Fluor® 594) (ab207016)
- Anti-CCR7 antibody [Y59] (Alexa Fluor® 555) (ab207018)
- Anti-CCR7 antibody [Y59] (Alexa Fluor® 568) (ab207019)
- Anti-CCR7 antibody [Y59] - Low endotoxin, Azide free (ab221209)
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab32527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).Can be blocked with CCR7 peptide (ab209386). For unpurified use at 1/5000. |
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| ICC/IF | 1/100. For unpurified use at 1/250. |
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| IP | 1/20. For unpurified use at 1/10. |
Target
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FunctionReceptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
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Tissue specificityExpressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1236 Human
- Entrez Gene: 12775 Mouse
- Entrez Gene: 287673 Rat
- Omim: 600242 Human
- SwissProt: P32248 Human
- SwissProt: P47774 Mouse
- Unigene: 370036 Human
- Unigene: 2932 Mouse
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Alternative names
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-CCR7 antibody [Y59] (ab32527)](http://a.abcam.com/ps/products/32/ab32527/Images/ab32527-6-ab32527ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR7 antibody [Y59] (ab32527)Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling CCR7 with purified ab32527 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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![Immunocytochemistry/ Immunofluorescence - Anti-CCR7 antibody [Y59] (ab32527)](http://a.abcam.com/ps/products/32/ab32527/Images/CCR7-Primary-antibodies-ab32527-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR7 antibody [Y59] (ab32527)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CCR7 with unpurified ab32527 at 1/1000. Cells were fixed with 4% PFA fixed (10 min) and permeabilized and blocked with 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody (ab32527, 1µg/ml) overnight at +4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (shown in green). Alexa Fluor® 594 WGA was used to label plasma membranes (shown in red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
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![Western blot - Anti-CCR7 antibody [Y59] (ab32527)](http://a.abcam.com/ps/products/32/ab32527/Images/ab32527-9-ab32527WB2.jpg)
Western blot - Anti-CCR7 antibody [Y59] (ab32527)All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/10000 dilution (purified)
Lane 1 : COS-1 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : RAW264.7 cell lysate
Lane 4 : PC-12 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 43 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Western blot - Anti-CCR7 antibody [Y59] (ab32527)](http://a.abcam.com/ps/products/32/ab32527/Images/ab32527-8-ab32527WB1.jpg)
Western blot - Anti-CCR7 antibody [Y59] (ab32527)All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/10000 dilution (purified)
Lane 1 : MCF-7 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 43 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Western blot - Anti-CCR7 antibody [Y59] (ab32527)](http://a.abcam.com/ps/products/32/ab32527/Images/ab32527wb.jpg)
Western blot - Anti-CCR7 antibody [Y59] (ab32527)All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/5000 dilution (unpurified)
Lane 1 : K562 cell lysate
Lane 2 : Daudi cell lysate
Predicted band size: 43 kDa
Observed band size: 48 kDa (why is the actual band size different from the predicted?) -
![Immunoprecipitation - Anti-CCR7 antibody [Y59] (ab32527)](http://a.abcam.com/ps/products/32/ab32527/Images/ab32527-7-ab32527IP1.jpg)
Immunoprecipitation - Anti-CCR7 antibody [Y59] (ab32527)ab32527 (purified) at 1/20 immunoprecipitating CCR7 in MCF-7 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/10,000) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
References
This product has been referenced in:
- Sonbul SN et al. Chemokine (C-C motif) receptor 7 (CCR7) associates with the tumour immune microenvironment but not progression in invasive breast carcinoma. J Pathol Clin Res 3:105-114 (2017). WB ; Human . Read more (PubMed: 28451459) »
- Subramanian S et al. TREM-1 associated macrophage polarization plays a significant role in inducing insulin resistance in obese population. J Transl Med 15:85 (2017). IHC-P ; Human . Read more (PubMed: 28454543) »
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