Recombinant
RabMAb

Anti-CCR7 antibody [Y59] (ab32527)

Abreviews (6)Q&A (8)Specific References (15)
Customer reviews and FAQs

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab32527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).Can be blocked with CCR7 peptide (ab209386).

For unpurified use at 1/5000.
ICC/IF 1/100.

For unpurified use at 1/250.
IP 1/20.

For unpurified use at 1/10.

Target

  • Function
    Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
  • Tissue specificity
    Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • BLR 2 antibody
      • BLR2 antibody
      • C C chemokine receptor type 7 antibody
      • C C CKR 7 antibody
      • C-C chemokine receptor type 7 antibody
      • C-C CKR-7 antibody
      • CC chemokine receptor 7 antibody
      • CC chemokine receptor type 7 antibody
      • CC CKR 7 antibody
      • CC-CKR-7 antibody
      • CCCKR7 antibody
      • CCR 7 antibody
      • CCR-7 antibody
      • Ccr7 antibody
      • CCR7_HUMAN antibody
      • CD 197 antibody
      • CD197 antibody
      • CD197 antigen antibody
      • CDw197 antibody
      • Chemokine (C C motif) receptor 7 antibody
      • Chemokine (C C) receptor 7 antibody
      • Chemokine C C motif receptor 7 antibody
      • Chemokine C C receptor 7 antibody
      • Chemokine receptor 7 antibody
      • Chemokine receptor 7-like protein antibody
      • CMKBR7 antibody
      • EBI 1 antibody
      • EBI1 antibody
      • Ebi1h antibody
      • EBV Induced G Protein Coupled Receptor 1 antibody
      • EBV-induced G-protein coupled receptor 1 antibody
      • Epstein Barr virus induced G protein coupled receptor antibody
      • Epstein Barr virus induced gene 1 antibody
      • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
      • EVI 1 antibody
      • EVI1 antibody
      • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
      • MGC108519 antibody
      • MIP 3 beta receptor antibody
      • MIP-3 beta receptor antibody
      • MIP3 Beta Receptor antibody
      see all

    Anti-CCR7 antibody [Y59] images

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR7 antibody [Y59] (ab32527)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR7 antibody [Y59] (ab32527)

      Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling CCR7 with purified ab32527 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR7 antibody [Y59] (ab32527)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR7 antibody [Y59] (ab32527)

      Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CCR7 with unpurified ab32527 at 1/1000. Cells were fixed with 4% PFA fixed (10 min) and permeabilized and blocked with 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody (ab32527, 1µg/ml) overnight at +4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (shown in green). Alexa Fluor® 594 WGA was used to label plasma membranes (shown in red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

    • Western blot - Anti-CCR7 antibody [Y59] (ab32527)
      Western blot - Anti-CCR7 antibody [Y59] (ab32527)
      All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/10000 dilution (purified)

      Lane 1 : COS-1 cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : RAW264.7 cell lysate
      Lane 4 : PC-12 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 43 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-CCR7 antibody [Y59] (ab32527)
      Western blot - Anti-CCR7 antibody [Y59] (ab32527)
      All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/10000 dilution (purified)

      Lane 1 : MCF-7 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 43 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-CCR7 antibody [Y59] (ab32527)
      Western blot - Anti-CCR7 antibody [Y59] (ab32527)
      All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/5000 dilution (unpurified)

      Lane 1 : K562 cell lysate
      Lane 2 : Daudi cell lysate


      Predicted band size : 43 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    • Immunoprecipitation - Anti-CCR7 antibody [Y59] (ab32527)
      Immunoprecipitation - Anti-CCR7 antibody [Y59] (ab32527)

      ab32527 (purified) at 1/20 immunoprecipitating CCR7 in MCF-7 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/10,000) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References for Anti-CCR7 antibody [Y59] (ab32527)

    This product has been referenced in:
    • Sonbul SN  et al. Chemokine (C-C motif) receptor 7 (CCR7) associates with the tumour immune microenvironment but not progression in invasive breast carcinoma. J Pathol Clin Res 3:105-114 (2017). WB ; Human . Read more (PubMed: 28451459) »
    • Subramanian S  et al. TREM-1 associated macrophage polarization plays a significant role in inducing insulin resistance in obese population. J Transl Med 15:85 (2017). IHC-P ; Human . Read more (PubMed: 28454543) »

    See all 15 Publications for this product

    Publishing research using ab32527? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab32527 Submit a question for ab32527

    14 Abreviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-CCR7 antibody [Y59]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF-7)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    7 µg
    Specification
    MCF-7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Jul 07 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CCR7 antibody [Y59]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (CT26-WT tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
    Permeabilization
    No
    Specification
    CT26-WT tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
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    Username

    Miss. Sylvie Colin

    Verified customer

    Submitted Jun 14 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CCR7 antibody [Y59]

     Below Average
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Sample
    Rat Tissue sections (nerve)
    Specification
    nerve
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jun 11 2013

    Hi,

    This seems very diluted?

    I have trouble to get a specific signal with this antibody doing immunohistochemistry. My negative control is tissue from a CCR7 knock out and I get signal even after using 1/5000 dilution. Have you te...

    Read More

    Thank you very much for contacting us.

    Rabbit monoclonal antibodies have very high affinity for their antigens, so they are often used more diluted than a comparable mouse monoclonal antibody would be. We don't have any data for htis antibody...

    Read More

    OK - I goofed up, sorry. The antibody that came lyophilized was actually the one that I got free on account of writing the abcam review for ab32527. But my question still stands as to what concentration the 100ul of ab32527 is at?

    Thanks for the email.

    I'm happy to provide the concentration. It's lot specific so when you have a chance please provide the lot number for that vial of ab32527.

    Thanks!

    Hi there, I have another Q regarding ab32527. It says it comes as a 100 ul solution but mine was actually lyophilized (or so it appeared) as such I resuspended it. Can you tell me the actual amount of antibody in mg quantity that was in the tube?

    Thanks for your email.

    According to information I have this product should arrive as a liquid (100ul). I'm sorry to ask a rather silly question here but did you centrifuge the vial prior to opening, in case all the liquid is in the cap?
    ...

    Read More

    Hi there - I just submitted the AbReview for Ab32527 for Flow but forgot to include the discount code. Sorry. I will be ordering a GLT-1 antibody today and will try and use the code.

    Thanks for your email.

    I hope you've been able to claim your free antibody. If not, please note I have added your discount code to your review for ab32527 so you should have no problem using that code to get your free primary now.

    I...

    Read More

    Flow Cytometry abreview for Anti-CCR7 antibody [Y59]

     Good
    Abreviews
    Application
    Flow Cytometry
    Sample
    Rat Cell (primary cortical cultures)
    Specification
    primary cortical cultures
    Fixation
    none
    Permeabilization
    No
    Gating Strategy
    live cells (no PI uptake)
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Aug 25 2012

    Yes, that sounds good. I just placed the order for the antibody in question and I will hopefully test it next week and provide feedback to you.


    I am very pleased to hear you would like to accept our offer and test ab32527 in flow cytometry. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF ANTIBODY OFF OF ORDER before the expiration date. To redeem this offer, please submit an A...

    Read More

    Inquiry: Could you please elaborate on why this antibody is noted as unsuitable for flow cytometry? What was it tested on and at what dilutions? Thanks!

    Thank you for your enquiry.

    I've looked and have not been able to find any information regarding flow cytometry testing and ab32527. It may be that this note on our datasheet is an error.

    If you'd like to test this antibody in flow ...

    Read More

    1-10 of 14 Abreviews or Q&A

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