Overview

  • Product nameAnti-CCR9 antibody [7E7]
    See all CCR9 primary antibodies
  • Description
    Rat monoclonal [7E7] to CCR9
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide:

    PTDFTSPIPNMADDYGSEST

    , corresponding to N terminal amino acids 3-22 of Mouse CCR9

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: RPMI 1640, 5% Foetal calf serum, Tissue culture supernatant
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone number7E7
  • MyelomaP3x63-Ag8.653
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/20.
Flow Cyt 1/20. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/20.

Target

  • RelevanceThe protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.
  • Cellular localizationCell membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • C C chemokine receptor D6 antibody
    • C C chemokine receptor type 9 antibody
    • C C CKR 9 antibody
    • CC Chemokine receptor CCR10 antibody
    • CC CKR 9 antibody
    • CCBP2 antibody
    • CCCKR9 antibody
    • CCR 9 antibody
    • CCR9 antibody
    • CDw199 antibody
    • CDw199 antigen antibody
    • Chemokine (C C motif) receptor 9 antibody
    • Chemokine (C C motif) receptor 9, isoform CRA_a antibody
    • Chemokine (C-C motif) receptor 9 isoform A antibody
    • Chemokine (C-C motif) receptor 9 isoform B antibody
    • Chemokine C C motif receptor 9 antibody
    • Chemokine C C receptor 10 antibody
    • Chemokine CC Motif Receptor 9 antibody
    • Chemokine receptor CCR 9 antibody
    • Chemokine receptor CCR9 antibody
    • Chemokine-binding protein D6 antibody
    • Cmkbr10 antibody
    • CMKBR9 antibody
    • G protein coupled receptor 28 antibody
    • GPR 28 antibody
    • GPR 9 6 antibody
    • GPR28 antibody
    • GPR96 antibody
    • OTTHUMP00000164653 antibody
    • OTTHUMP00000164654 antibody
    see all

Anti-CCR9 antibody [7E7] images

  • ab52590 staining CCR9 in Mouse brain tissue sections by Immunocytochemistry/ Immunofluorescence.

    Red = CCR9

    Green = IgA

    Blue = CD3

  • Overlay histogram showing K562 cells stained with ab52590 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52590, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-CCR9 antibody [7E7] (ab52590)

This product has been referenced in:
  • Pabst O  et al. Chemokine receptor CCR9 contributes to the localization of plasma cells to the small intestine. J Exp Med 199:411-6 (2004). Read more (PubMed: 14744993) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"