SpecificityThe antibody is specific to CCR9. It does not cross react with other G protein coupled receptor 1 family members.
Tested applicationsSuitable for:
WB, ICC, Flow Cytmore details Unsuitable for:
IHC or IP
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CCR9 (N terminal). The exact sequence is proprietary. A synthetic peptide corresponding to an extracellular domain of human CCR9.
K562 and Daudi cell lysates.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notesIs unsuitable for IHC or IP.
RelevanceThe protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.
Maeda S et al. Molecular cloning and expression analysis of the canine chemokine receptor CCR9. Vet Immunol Immunopathol145:534-9 (2012).
Read more (PubMed: 22209203) »
Shen X et al. CC Chemokine Receptor 9 Enhances Proliferation in Pancreatic Intraepithelial Neoplasia and Pancreatic Cancer Cells. J Gastrointest Surg : (2009).
WB, Flow Cyt
Read more (PubMed: 19756884) »