Overview

  • Product nameAnti-CCRL2 antibody
    See all CCRL2 primary antibodies
  • Description
    Rabbit polyclonal to CCRL2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (LSAQLVPSLC SAVFVIGVLD NLLVVLILVK YKGLKRVENI YLLNLAVSNL) of Human CCRL2 (NP_003956).

  • Positive control
    • 721_B Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionReceptor for CCL19 and chemerin/RARRES2. Does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. Plays a critical role for the development of Th2 responses.
  • Tissue specificityExpressed abundantly in immunal tissues such as spleen, fetal liver, lymph node and bone marrow. Strong expression also in lung and heart. Expressed in almost all hematopoietic cells including monocytes, macrophages, PMNs, T-cells (both CD4+ and CD8+), monocyte-derived iDCs, NK cells, and CD34+ progenitor cells. B cells expressed isoform 1 but not isoform 2. Up-regulated on synovial neutrophils of rheumatoid arthritis patients.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • DomainLacks the conserved DRYLAIV motif in the second intracellular loop that is required for signaling of functional chemokine receptors.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C chemokine receptor-like 2 antibody
    • CCRL2 antibody
    • CCRL2_HUMAN antibody
    • Chemokine (C-C motif) receptor like 2 antibody
    • Chemokine receptor CCR11 antibody
    • Chemokine receptor X antibody
    • CKRX antibody
    • CRAM A antibody
    • CRAM B antibody
    • CRAMA antibody
    • CRAMB antibody
    • FLJ55815 antibody
    • HCR antibody
    • MGC116710 antibody
    • MGC34104 antibody
    • Putative MCP 1 chemokine receptor antibody
    • Putative MCP-1 chemokine receptor antibody
    see all

Anti-CCRL2 antibody images

  • Anti-CCRL2 antibody (ab85224) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : ~39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : ~70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CCRL2 antibody (ab85224)

ab85224 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"