Anti-CCRL2 antibody (ab88632)
Key features and details
- Mouse polyclonal to CCRL2
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-CCRL2 antibody
See all CCRL2 primary antibodies -
Description
Mouse polyclonal to CCRL2 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human CCRL2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_003956 -
Positive control
- RAW 264.7 cell lyate, NIH/3T3 cell lysate, transfected 293 cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
|
|
Flow Cyt | (5) |
Use at an assay dependent concentration.
ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. |
Flow Cyt
Use at an assay dependent concentration. ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
Receptor for CCL19 and chemerin/RARRES2. Does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. Plays a critical role for the development of Th2 responses. -
Tissue specificity
Expressed abundantly in immunal tissues such as spleen, fetal liver, lymph node and bone marrow. Strong expression also in lung and heart. Expressed in almost all hematopoietic cells including monocytes, macrophages, PMNs, T-cells (both CD4+ and CD8+), monocyte-derived iDCs, NK cells, and CD34+ progenitor cells. B cells expressed isoform 1 but not isoform 2. Up-regulated on synovial neutrophils of rheumatoid arthritis patients. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Domain
Lacks the conserved DRYLAIV motif in the second intracellular loop that is required for signaling of functional chemokine receptors. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 9034 Human
- Entrez Gene: 54199 Mouse
- Omim: 608379 Human
- SwissProt: O00421 Human
- SwissProt: O35457 Mouse
- Unigene: 535713 Human
- Unigene: 7336 Mouse
-
Alternative names
- C-C chemokine receptor-like 2 antibody
- CCRL2 antibody
- CCRL2_HUMAN antibody
see all
Images
-
FACS analysis of negative control 293 cells (black) and CCRL2-expressing 293 cells (green) using ab88632.
-
Anti-CCRL2 antibody (ab88632) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg
Predicted band size: 40 kDa -
Anti-CCRL2 antibody (ab88632) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg
Predicted band size: 40 kDa -
All lanes : Anti-CCRL2 antibody (ab88632) at 1 µg/ml
Lane 1 : CCRL2-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 40 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
Datasheet download
References (4)
ab88632 has been referenced in 4 publications.
- Zhang Y et al. Mycobacterium tuberculosis Heat-Shock Protein 16.3 Induces Macrophage M2 Polarization Through CCRL2/CX3CR1. Inflammation 43:487-506 (2020). PubMed: 31748849
- Liang Z et al. Chemerin-induced macrophages pyroptosis in fetal brain tissue leads to cognitive disorder in offspring of diabetic dams. J Neuroinflammation 16:226 (2019). PubMed: 31733653
- Zhang Y et al. Chemerin suppresses neuroinflammation and improves neurological recovery via CaMKK2/AMPK/Nrf2 pathway after germinal matrix hemorrhage in neonatal rats. Brain Behav Immun 70:179-193 (2018). PubMed: 29499303
- Li L et al. Expression of chemerin and its receptors in rat testes and its action on testosterone secretion. J Endocrinol 220:155-63 (2014). PubMed: 24301613