Anti-CCRL2 antibody (ab88632)

Overview

  • Product nameAnti-CCRL2 antibodySee all CCRL2 primary antibodies ...
  • Description
    Mouse polyclonal to CCRL2
  • Tested applicationsWB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length Human protein (NP_003956)

  • Positive control
    • RAW 264.7 cell lyate, NIH/3T3 cell lysate, transfected 293 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab88632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
Flow Cyt Use at an assay dependent dilution.

Target

  • FunctionReceptor for CCL19 and chemerin/RARRES2. Does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. Plays a critical role for the development of Th2 responses.
  • Tissue specificityExpressed abundantly in immunal tissues such as spleen, fetal liver, lymph node and bone marrow. Strong expression also in lung and heart. Expressed in almost all hematopoietic cells including monocytes, macrophages, PMNs, T-cells (both CD4+ and CD8+), monocyte-derived iDCs, NK cells, and CD34+ progenitor cells. B cells expressed isoform 1 but not isoform 2. Up-regulated on synovial neutrophils of rheumatoid arthritis patients.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • DomainLacks the conserved DRYLAIV motif in the second intracellular loop that is required for signaling of functional chemokine receptors.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C chemokine receptor-like 2 antibody
    • CCRL2 antibody
    • CCRL2_HUMAN antibody
    • Chemokine (C-C motif) receptor like 2 antibody
    • Chemokine receptor CCR11 antibody
    • Chemokine receptor X antibody
    • CKRX antibody
    • CRAM A antibody
    • CRAM B antibody
    • CRAMA antibody
    • CRAMB antibody
    • FLJ55815 antibody
    • HCR antibody
    • MGC116710 antibody
    • MGC34104 antibody
    • Putative MCP 1 chemokine receptor antibody
    • Putative MCP-1 chemokine receptor antibody
    see all

Anti-CCRL2 antibody images

  • FACS analysis of negative control 293 cells (black) and CCRL2-expressing 293 cells (green) using ab88632.
  • Anti-CCRL2 antibody (ab88632) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 40 kDa
  • Anti-CCRL2 antibody (ab88632) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg

    Predicted band size : 40 kDa
  • All lanes : Anti-CCRL2 antibody (ab88632) at 1 µg/ml

    Lane 1 : CCRL2-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-CCRL2 antibody (ab88632)

ab88632 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Dog Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive cells
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Submitted Nov 14 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive monocytes
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Submitted Apr 27 2012

Application Flow Cytometry
Sample Mouse Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive monocytes
Username

Abcam user community

Verified customer

Submitted Apr 19 2012

Thank you for submitting an Abreview of ab88632. As you may have noticed, your review will be published shortly on our website.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive macrophages
Username

Abcam user community

Verified customer

Submitted Apr 13 2012

Application Flow Cytometry
Sample Human Cell (macrophages)
Specification macrophages
Permeabilization No
Gating Strategy alive cells
Username

Abcam user community

Verified customer

Submitted Jan 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"