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Prokaryotic recombinant fusion protein corresponding to the external of the CD10 glycoprotein
We have mixed customer feedback about the mouse specificity, which is why we no longer guarantee this species. Contact scientific support team for any inquiry.
This antibody works well when used in combination with ab972 - Antigen retrieval solution, heat mediated high pH 9.5.
Our Abpromise guarantee covers the use of ab951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 85 kDa. PubMed: 26074811|
|IHC-P||1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, incubate for 30-60 minutes at RT. With high pH retrieval solutions, some tissues may require an avidin-biotin block. Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult. It is therefore recommended that a light hematoxylin stain be applied. Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-).|
Paraffin embedded renal cell carcinoma tissue stained for CD10 with ab951 (1/25 dilution) in immunohistochemical analysis.
ab951 at 1/20 dilution staining CD10 in MDA-MB-231 human breast cancer cells grown in mouse by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C and then incubated with ab951 for 17 hours at 4°C. A rabbit polyclonal to mouse Ig, diluted 1/200 was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"