Overview

  • Product nameAnti-CD10 antibody [56C6]
    See all CD10 primary antibodies
  • Description
    Mouse monoclonal [56C6] to CD10
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Cat, Dog, Human
    Predicted to work with: Mouse
  • Immunogen

    Prokaryotic recombinant fusion protein corresponding to the external of the CD10 glycoprotein

  • Positive control
    • Tonsil, kidney or lymphoblastic leukemia.
  • General notes

    We have mixed customer feedback about the mouse specificity, which is why we no longer guarantee this species. Contact scientific support team for any inquiry.

    This antibody works well when used in combination with ab972 - Antigen retrieval solution, heat mediated high pH 9.5.

Properties

Applications

Our Abpromise guarantee covers the use of ab951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 85 kDa.
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, incubate for 30-60 minutes at RT. With high pH retrieval solutions, some tissues may require an avidin-biotin block. Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult. It is therefore recommended that a light hematoxylin stain be applied. Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-).
IHC-Fr 1/25 - 1/50. For an ABC system, incubate for 30-60 minutes at RT.

Target

  • FunctionThermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
  • Sequence similaritiesBelongs to the peptidase M13 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Atriopeptidase antibody
    • CALLA antibody
    • CD10 antibody
    • CD10 antigen antibody
    • Common acute lymphocytic leukemia antigen antibody
    • DKFZp686O16152 antibody
    • EC 3.4.24.11 antibody
    • Enkephalinase antibody
    • EPN antibody
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
    • Membrane metallo endopeptidase antibody
    • Membrane metallo endopeptidase variant 1 antibody
    • Membrane metallo endopeptidase variant 2 antibody
    • Membrane metalloendopeptidase antibody
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
    • Membrane metalloendopeptidase variant 1 antibody
    • Membrane metalloendopeptidase variant 2 antibody
    • MGC126681 antibody
    • MGC126707 antibody
    • MME antibody
    • NEP antibody
    • NEP_HUMAN antibody
    • Neprilysin antibody
    • neprilysin-390 antibody
    • neprilysin-411 antibody
    • Neutral endopeptidase 24.11 antibody
    • Neutral endopeptidase antibody
    • Neutral endopeptidase, membrane-associated antibody
    • SFE antibody
    • Skin fibroblast elastase antibody
    see all

Anti-CD10 antibody [56C6] images

  • ab951 at 1/20 dilution staining CD10 in MDA-MB-231 human breast cancer cells grown in mouse by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C and then incubated with ab951 for 17 hours at 4°C. A rabbit polyclonal to mouse Ig, diluted 1/200 was used as the secondary antibody.

    See Abreview

References for Anti-CD10 antibody [56C6] (ab951)

This product has been referenced in:
  • D'Souza Y  et al. Characterization of Aldh2 (-/-) mice as an age-related model of cognitive impairment and Alzheimer's disease. Mol Brain 8:27 (2015). WB, Dot Blot ; Mouse . Read more (PubMed: 25910195) »
  • Kinoshita M  et al. Different spatial distribution between germinal center B and non-germinal center B primary central nervous system lymphoma revealed by magnetic resonance group analysis. Neuro Oncol 16:728-34 (2014). Human . Read more (PubMed: 24497406) »

See all 17 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Uterus)
Permeabilization Yes - 0.5% Triton/PBS
Specification Uterus
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

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Verified customer

Submitted Jul 29 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (DLCBL cell line WSUDLCL2 xenograft)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization No
Specification DLCBL cell line WSUDLCL2 xenograft
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative Formaldehyde
Username

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Verified customer

Submitted Dec 16 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mammary gland)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate 10mM pH=6.0
Permeabilization Yes - PBS with 3% Triton
Specification mammary gland
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

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Verified customer

Submitted Aug 04 2015


The IgG concentration of ab951 is 7.2µg/mL.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

I am sorry that these protocol details did not yield the expected results. The details p...

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Thank you for contacting us and your interest in our products.

The concentration of the Anti-CD10 antibody [56C6] (ab951), lot number GR38260-2 is 7.2µg/mL.

I hope this information has been of help. If you have any further que...

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Thank you for contacting us.

I am sorry that this product has not been performing as it should in mouse.This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product i...

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Thank you for contacting us.

The IgG concentration for lot GR79429-1 is 7.2µg/mL. For IHC, the antibody should be used at a concentration of 1/20 - 1/50. The antibody was validated for IHC with a sensitive polymer detection system, so if you...

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Thank you for contacting Abcam.

I think you are correct that the band that you are seeing at 100kDa is correct as based on the Swissprot sequence http://www.uniprot.org/uniprot/Q61391, there are q few glycosylation sites on this protein that ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"