The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 10µl for 106 cells or 100 µl of whole blood.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Sequence similaritiesBelongs to the peptidase M13 family.
A typical staining pattern of granulocytes with ab27384.
References for Anti-CD10 antibody [B-E3] (Phycoerythrin) (ab27384)
This product has been referenced in:
Pan C et al. CD10 is a key enzyme involved in the activation of tumor-activated peptide prodrug CPI-0004Na and novel analogues: implications for the design of novel peptide prodrugs for the therapy of CD10+ tumors. Cancer Res63:5526-31 (2003).
Read more (PubMed: 14500390) »