The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. 20 µl for 100 µl sample is recommended.
ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionMajor glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
Tissue specificityEndoglin is restricted to endothelial cells in all tissues except bone marrow.
Involvement in diseaseDefects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
Flow cytometry analysis of rat bone marrow mesenchymal stem cells, staining CD105 (blue histograms) with ab18278.
Cells were cultured in were isolated from young (YBM-MSC) and old (OBM-MSC) Sprague Dawley rats. The sample was incubated with the primary antibody (1/100) for 30 minutes on ice.
References for Anti-CD105 antibody [MEM-226] (FITC) (ab18278)
This product has been referenced in:
Hong L et al. The effects of 17-ß estradiol on enhancing proliferation of human bone marrow mesenchymal stromal cells in vitro. Stem Cells Dev20:925-31 (2011).
Read more (PubMed: 20735179) »
Asumda FZ & Chase PB Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol12:44 (2011).
Read more (PubMed: 21992089) »